Confocal Raman Spectroscopy: in Vivo Measurement of Physiological Skin Parameters

Not Applicable

Completed

• Conditions: Disorder of Cholesterol Metabolism; Dermatologic Disorders
• Interventions: Procedure: Venous blood collection; Procedure: Raman spectroscopy

• Registration Number: NCT02854475
• Lead Sponsor: University of Vienna

Brief Summary

The purpose of this study is to

I. determine several skin parameters, for example natural moisturizing factor (NMF) and cholesterol, with Raman spectroscopy in vivo and

II. compare the results with the corresponding cholesterol levels measured in venous blood.

Detailed Description

In vivo Raman experiments will be carried out weekly on voluntary participants using a RiverDiagnostics gen2-SCA Skin Composition Analyzer (RiverDiagnostics, Rotterdam, Netherlands). All experiments will be carried out on the volar forearm. Semi-quantitative concentration profiles for several skin parameters will be determined, for example natural moisturizing factor (NMF) and cholesterol.

Additionally the total cholesterol level will be detected by venous blood sample analysis. The blood samples will be taken weekly within two weeks, similar to the Raman measurements.

Study Timeline

• Status Verified: 2016-07
• Study Start: 2016-07
• Primary Completion: 2016-07
• Study Completion: 2016-07
• Study First Submitted: 2016-07-27
• Study First Submitted QC: 2016-07-29
• Last Update Submitted: 2016-07-29
• Study First Posted: 2016-08-03
• Last Update Posted: 2016-08-03

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 15

Inclusion Criteria

• Provision of subject informed consent
• Female or male aged between 18 and 65 years

Exclusion Criteria

• Pregnancy
• Chronic skin conditions

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
Proband group	Venous blood collection	Raman spectroscopy and venous blood collection
Proband group	Raman spectroscopy	Raman spectroscopy and venous blood collection

Primary Outcome Measures

Name	Time	Method
Correlation of the fasting total cholesterol levels measured in skin with confocal Raman spectroscopy and in venous blood collected by venipuncture	2 weeks

Secondary Outcome Measures

Name	Time	Method
Concentration curves of various skin substances, such as NMF, urea and water as evidence for the eligibility of confocal Raman spectroscopy for the in vivo measurement of skin	2 weeks