Markers of Carnitine Acetyltransferase (CrAT) Protein Activity and Carnitine Availability
- Conditions
- Metabolism and Nutrition DisorderMetabolic Disturbance
- Interventions
- Other: Exercise metabolism
- Registration Number
- NCT03697928
- Lead Sponsor
- Maastricht University
- Brief Summary
This study aims to measure skeletal muscle metabolism in vivo during exercise using the non-invasive Magnetic Resonance Spectroscopy (MRS) technique. Specifically, phosphocreatine (PCr) kinetic and Acetylcarnitine levels will be determined at resting, during exercise and during the recovery post exercise. The target population is adult healthy men, with a wide range of maximal physical capacity.
- Detailed Description
Skeletal muscle mitochondrial activation during the transition from resting metabolism to exercise may modulated by CrAT activity. Furthermore, dynamic changes in acetylcarnitine during the transition from exercise to resting metabolism (acetylcarnitine off-kinetic) may also reflect the action of CrAT protein. To date, the only possibility to get information on CrAT activity is by taking a muscle biopsy and perform enzyme activity assays ex vivo. By dynamic MRS scanning, it might be possible to deduce information that reflects CrAT activity, thereby making the biopsy sampling unnecessary.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- Male
- Target Recruitment
- 13
- Males, Generally healthy
- Age 18-40 years old
- BMI 18 - 25 kg/m2
- No medication use that interfere with the aims of the study
- Stable level of daily physical activity or training for at least 6 months
- Females
- Regular smokers, drug abuse
- Participation in other studies
- Weight gain/loss > 3 kg in the last 6 months
- Contraindications for MRS scans:
- Vegetarian eating behavior
Study & Design
- Study Type
- OBSERVATIONAL
- Study Design
- Not specified
- Arm && Interventions
Group Intervention Description CrAT subjects Exercise metabolism 13 healthy adult men, with a wide range of maximal aerobic capacity will be included. Subjects will be classified as trained, physical active and untrained according to their VO2max. Subject will perform one-legged knee extension and flexion exercise inside the MRS scanner and cycling outside the scanner
- Primary Outcome Measures
Name Time Method Skeletal muscle PCr on-kinetic during exercise and carnitine acetyltransferase (CrAT) protein content/activity MRS+Exercise test day 2 The correlation between skeletal muscle PCr on-kinetic during one-legged exercise and CrAT protein activity will be determined.
- Secondary Outcome Measures
Name Time Method Skeletal muscle acetylcarnitine recovery after one-legged exercise and carnitine acetyltransferase (CrAT) protein content/activity MRS+Exercise test day 2 The correlation between skeletal muscle acetylcarnitine off-kinetic after one-legged exercise and CrAT protein activity will be determined.
Skeletal muscle oxygen availability MRS test day 1 in vivo assessment of oxygen availability in muscle tissue upon hypoxic stimulus during resting
Skeletal muscle CrAT protein activity, from muscle biopsy, and PCr breakdown rate during one-legged exercise MRS+Exercise test day 1 The correlation between CrAT protein activity and PCr hydrolysis/breakdown will be determined
Skeletal muscle acetylcarnitine formation capacity upon exercise and total carnitine availability in skeletal muscle MRS+Exercise test day 2 The correlation between acetylcarnitine formation upon exercise and total carnitine availability will be determined
Skeletal muscle mitochondrial function in vitro MRS+Exercise test day 2 The association between Skeletal muscle mitochondrial function in vitro and in vivo
Trial Locations
- Locations (1)
Maastricht University
🇳🇱Maastricht, Limburg, Netherlands