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Plasma Therapy of COVID-19 in Severely Ill Patients

Phase 2
Completed
Conditions
SARS-CoV-2 Infection
Interventions
Biological: Convalescent Plasma (anti-SARS-CoV-2 plasma)
Biological: Non-convalescent Plasma (control plasma)
Registration Number
NCT04359810
Lead Sponsor
Max O'Donnell
Brief Summary

This randomized blinded multi-center phase 2 trial will assess the efficacy and safety of anti-SARS-CoV-2 convalescent plasma among adults with severe COVID-19. Adults ≥18 years of age may participate. A total of 219 eligible subjects will be randomized in a 2:1 ratio to receive either high-titer anti-SARS-CoV-2 plasma or non-convalescent fresh frozen plasma (control plasma).

Detailed Description

There are few effective therapies for coronavirus disease 2019 (COVID-19) associated with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Exposure to viruses results in an adaptive immune response that commonly include antibodies with neutralization activity. Plasma from subjects who have recovered from viral infections has been used to both prevent or treat disease. Notable examples of the successful use of convalescent plasma (CP) include influenza, measles, Argentine hemorrhagic fever, Middle East respiratory syndrome (MERS), Ebola and severe acute respiratory syndrome (SARS-CoV). Small clinical trials and observational studies of CP therapy in patients with COVID-19 have suggested a possible clinical benefit.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
223
Inclusion Criteria
  • Willing and able to provide written informed consent prior to performing study procedures or have a legally authorized representative available to do so.
  • Age ≥18 years
  • Evidence of SARS-CoV-2 infection by PCR test of naso-/oro-pharyngeal swab or tracheal aspirate sample within 14 days of randomization
  • Peripheral capillary oxygen saturation (SpO2) ≤ 94% on room air or requiring supplemental oxygen, non-invasive or invasive mechanical ventilation at screening
  • Evidence of infiltrates on chest radiography
  • Females of childbearing age and males, must be willing to practice an effective contraceptive method or remain abstinent during the study period.
Exclusion Criteria
  • Participation in another clinical trial of anti-viral agent(s) for COVID-19

  • Receipt of any anti-viral agent(s) with possible activity against SARS-CoV-2 <24 hours prior to study drug administration [1]

  • Mechanically ventilated (including veno-venous (VV)-ECMO) ≥ 5 days

  • Severe multi-organ failure

  • History of prior reactions to transfusion blood products meeting definitive case definition criteria, at least severe severity, and probable or definite imputability per National Healthcare Safety Network (NHSN)/Centers for Disease Control and Prevention (CDC) criteria

  • Known Immunoglobulin A (IgA) deficiency

  • Females who are pregnant

    1. Use of remdesivir as treatment for COVID-19 is permitted.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Convalescent Plasma (anti-SARS-CoV-2 plasma)Convalescent Plasma (anti-SARS-CoV-2 plasma)Convalescent plasma (1 unit; \~200-250 mL) collected from a volunteer who recovered from COVID-19 disease
Non-convalescent Plasma (control plasma)Non-convalescent Plasma (control plasma)Non-convalescent plasma (1 unit; \~200-250 mL) of standard plasma collected prior to December 2019
Primary Outcome Measures
NameTimeMethod
Number of Participants With Day 28 Severity Outcomes Using a 7-point Ordinal Scale of Clinical StatusUp to 28 days

Compare the day 28 severity outcome amongst the anti-SARS-CoV-2 convalescent plasma and non-convalescent plasma groups using a 7-point ordinal scale of clinical status. Seven-point ordinal scale for clinical assessment ranges from

1 and 2: Not hospitalized 3: Hospitalized, not requiring supplemental oxygen 4: Hospitalized, requiring supplemental oxygen 5: Hospitalized, requiring high-flow oxygen therapy or noninvasive mechanical ventilation 6: Hospitalized, requiring IMV, ECMO, or both 7: Dead Higher number means worse outcome

Secondary Outcome Measures
NameTimeMethod
Proportion of SARS-CoV-2 PCR PositivityUp to 14 days

Compare the proportion and duration of SARS-CoV-2 PCR positivity (via RT-PCR) amongst the anti-SARS-CoV-2 convalescent plasma and non-convalescent plasma groups at days 0, 7, and 14.

Levels of SARS-CoV-2 RNAUp to 14 days

Compare levels of SARS-CoV-2 RNA amongst the anti-SARS-CoV-2 convalescent plasma and non-convalescent plasma groups at days 0, 7, and 14.

Duration of Need for Supplemental OxygenUp to 28 days

Compare duration of need for supplemental oxygen and/or mechanical ventilation amongst the anti-SARS-CoV-2 convalescent plasma and non-convalescent plasma groups.

Duration of HospitalizationUp to 28 days

Compare duration of hospitalization amongst the anti-SARS-CoV-2 convalescent plasma and non-convalescent plasma groups.

28-day MortalityUp to 28 days

Compare up to 28-day mortality amongst the anti-SARS-CoV-2 convalescent plasma and non-convalescent plasma groups.

Time-to-clinical Improvement In-hospitalUp to 28 days

Compare time-to-clinical improvement in-hospital amongst the anti-SARS-CoV-2 convalescent plasma and non-convalescent plasma groups, defined as the time to either an improvement of clinical status or alive discharge from the hospital, whichever comes first.

Host Genetic Differences at Day 0Day 0

To assess for host genetic differences at Day 0 amongst the anti-SARS-CoV-2 convalescent plasma and non-convalescent plasma groups.

Host Transcriptomic Differences at Days 0,7,14Up to 14 days

To assess for host transcriptomic differences at Day 0,7,14 amongst the anti-SARS-CoV-2 convalescent plasma and non-convalescent plasma groups.

Trial Locations

Locations (4)

Columbia University Irving Medical Center/NYP

🇺🇸

New York, New York, United States

Hospital Federal dos Servidores do Estado

🇧🇷

Rio De Janeiro, Brazil

Hospital Geral de Nova Iguaçu

🇧🇷

Rio De Janeiro, Brazil

Instituto Nacional de Infectologia Evandro Chagas

🇧🇷

Rio De Janeiro, Brazil

Columbia University Irving Medical Center/NYP
🇺🇸New York, New York, United States
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