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Organ protection by noble gases – Helium induced Early and Late Preconditioning (HELP) in human endothelium.

Recruiting
Conditions
Healthy volunteers.
Registration Number
NL-OMON29256
Lead Sponsor
dr. B. Preckel, MD, MA, DEAA, Department of Anesthesiology, Academic Medical CenterUniversity of Amsterdam, Meibergdreef 9Postbus 22660 H1Z-120, 1100 DD AmsterdamThe NetherlandsPhone: +31 20 5662162, +31 20 5662533 (Secr.)Fax:+31 20 6979441, e-mail : b.preckel@amc.uva.nl
Brief Summary

/A

Detailed Description

Not available

Recruitment & Eligibility

Status
Recruiting
Sex
Not specified
Target Recruitment
55
Inclusion Criteria

Healthy volunteers aged 18-65 years, informed consent.

Exclusion Criteria

1. Alcohol consumption;

2. untreated arterial hypertension;

Study & Design

Study Type
Interventional
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
The present application of helium seeks to investigate the possibility to precondition human endothelium with helium. This will be done by measurement of forearm blood flow using the venous occlusion plethysmography model. Primary end-point is blood flow and reactive hyperemia before and after ischemia reperfusion after stimulation with either serotonin as an endothelium-dependent vasodilator or sodium nitroprusside as an endothelium-independent vasodilator.
Secondary Outcome Measures
NameTimeMethod
Secondary parameters will be measurement as endothelial adhesion molecules on leukocytes.
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