Association Between SNP of SP-D and Susceptibility and Prognosis of Infectious Keratitis

Completed

• Conditions: Infectious Keratitis
• Interventions: Other: Sequencing; Drug: Treatment of bacterial keratitis; Drug: Treatment of fungal keratitis; Drug: Treatment of viral keratitis; Diagnostic Test: corneal scraping

• Registration Number: NCT04213313
• Lead Sponsor: Second Affiliated Hospital, School of Medicine, Zhejiang University

Brief Summary

This study explores the association between single nucleotide polymorphism (Met11Thr) of surfactant protein D(SP-D) and susceptibility and prognosis of infectious keratitis.Met11Thr of SP-D of patients with keratitis and normal controls were compared.Allele and genotype frequencies of patients with keratitis and normal controls were determined by polymerase chain reaction with sequence specific primers(PCR-SSPs) .SP-D gene polymorphism of patients with keratitis and normal controls was detected by Sanger sequencing

Detailed Description

Surfactant protein D(SP-D) plays an important role in the innate immune system of the eye in the lacrimal duct, cornea and conjunctiva.The single lipopolysaccharide on the surface of bacteria and the receptors on the surface of immune cells are linked by SP-D ,which acts as a bridge to neutralize viruses in the innate immune defense system and assists other immune cells in clearing bacteria and fungi.SP-D can also participate in the acquired immune response after combining with lipopolysaccharide on the surface of microorganisms.Some single nucleotide polymorphisms of SP - D can not only affect the plasma level of SP - D, which leads to the differences of pathogen defense capability, but also can affect the space configuration and structure of carbohydrate recognition domain of SP-D, causing the change of ability of SP - D combined with pathogens to influence its defensive role.The concentration of SP - D in ocular surface is closely related to the occurrence and progression of infectious keratitis.Association between single nucleotide polymorphisms of SP-D and the occurrence and development of infectious keratitis has yet to be confirmed by research.

Study Timeline

• Study Start: 2016-10-01
• Primary Completion: 2018-06-30
• Study Completion: 2019-06-30
• Status Verified: 2019-12
• Study First Submitted: 2019-12-24
• Study First Submitted QC: 2019-12-24
• Study First Posted: 2019-12-30
• Last Update Submitted: 2020-01-04
• Last Update Posted: 2020-01-07

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 145

Inclusion Criteria

• Clinical diagnosis of Infectious Keratitis.
• Laboratory diagnosis of Infectious Keratitis.

Exclusion Criteria

• Patients with topical or systemic long-term use of hormones, interferon, or immunosuppressive therapy.
• Patients with basic diseases such as diabetes, autoimmune disease, sjogren's syndrome, blepharitis, eyelid defect or incomplete eyelid closure.
• Patients with history of corneal myopia laser, cataract surgery and vitrectomy, or history of serious ocular trauma and drug allergies.

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
Control Group	Sequencing	health volunteers
Infectious Keratitis Group	Treatment of bacterial keratitis	Infectious corneal patients
Infectious Keratitis Group	Treatment of fungal keratitis	Infectious corneal patients
Infectious Keratitis Group	Treatment of viral keratitis	Infectious corneal patients
Infectious Keratitis Group	corneal scraping	Infectious corneal patients
Infectious Keratitis Group	Sequencing	Infectious corneal patients

Primary Outcome Measures

Name	Time	Method
Genotype	before the treatment	Genotype of SNP（rs721917）:( Met /Met , Thr/Thr and Met/Thr)
Base type	before the treatment	Base type of SNP（rs721917）:(Met and Thr)

Secondary Outcome Measures

Name	Time	Method
Grading of Keratitis	before the treatment	Grading of Keratitis： 1. cornea:transparency ,edema,turbidity,perforation; 2. conjunctiva: hyperaemia; 3. blephar:inflammation

Trial Locations

Locations (1)

Department of Ophthalmology, The Second Affiliated Hospital, Zhejiang University School of Medicine; 🇨🇳 Hangzhou, Zhejiang, China