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Fibroblast Growth Factor 23 and Sclerostin in Relation to Calcium in COVID-19 Patients

Completed
Conditions
COVID-19
Interventions
Diagnostic Test: Calcium
Diagnostic Test: Fibroblast growth factor 23
Diagnostic Test: Sclerostin
Diagnostic Test: parathyroid hormone
Registration Number
NCT05275491
Lead Sponsor
Aswan University Hospital
Brief Summary

Hypocalcemia is associated with COVID-19 patients and is linked to poor prognosis, Fibroblast growth factor 23 (FGF23) and Sclerostin inhibit vitamin D activation and are linked to hypocalcemia.

Levels of FGF23 and Sclerostin in COVID-19 patients will be detected and correlated to calcium levels.

Detailed Description

COVID 19 is a current worldwide pandemic caused by severe acute respiratory syndrome coronavirus 2 "SARS-CoV-2". Hypovitaminosis D and hypocalcaemia have been reported to be associated with COVID 19 infection, they are considered good biomarkers of clinical severity of COVID 19. Poor prognosis related to hypocalcemia is increased in elderly patients.

Fibroblast growth factor 23 and sclerostin are secreted from the bone, they decrease active vitamin D levels. Their levels and their relation to calcium levels in COVID-19 patients will be detected.

Recruitment & Eligibility

Status
COMPLETED
Sex
Male
Target Recruitment
66
Inclusion Criteria
  • Positive COVID 19 (Mild to moderate - severe to critical).
Exclusion Criteria
  • known diagnosis of CKD.
  • Known parathyroid disease.
  • Use of phosphate binder therapy within the past 3 months.
  • Use of calcium therapy within the past 3 months
  • Treatment with 25(OH) vitamin D or 1,25 (OH)(2) D
  • Underlying metabolic bone disease.
  • Underlying renal phosphate wasting disorder.

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Arm && Interventions
GroupInterventionDescription
Normal male subjects (Control)Calcium-
Severe to critical COVID 19 patientsparathyroid hormoneAccording to COVID 19 treatment guidelines of National Institutes of Health (NIH): Severe illness that show SpO2 \< 94% on room air, (PaO2/FiO2) \<300 mm Hg \& lung infiltration \> 50% with respiratory rate \> 30 breath/min. Critical illness that show respiratory failure, septic shock or multiorgan failure.
Severe to critical COVID 19 patientsFibroblast growth factor 23According to COVID 19 treatment guidelines of National Institutes of Health (NIH): Severe illness that show SpO2 \< 94% on room air, (PaO2/FiO2) \<300 mm Hg \& lung infiltration \> 50% with respiratory rate \> 30 breath/min. Critical illness that show respiratory failure, septic shock or multiorgan failure.
Mild to moderate COVID 19 patientsCalciumAccording to COVID 19 treatment guidelines of National Institutes of Health (NIH): Mild illness that show symptoms like fever, cough, nausea, vomiting, sore throat, loss of taste \& smell but don't show dyspnea or abnormal chest imaging. Moderate illness that show clinical or radiological lower respiratory disease with SpO2 \> 94% on room air.
Mild to moderate COVID 19 patientsSclerostinAccording to COVID 19 treatment guidelines of National Institutes of Health (NIH): Mild illness that show symptoms like fever, cough, nausea, vomiting, sore throat, loss of taste \& smell but don't show dyspnea or abnormal chest imaging. Moderate illness that show clinical or radiological lower respiratory disease with SpO2 \> 94% on room air.
Normal male subjects (Control)Sclerostin-
Mild to moderate COVID 19 patientsFibroblast growth factor 23According to COVID 19 treatment guidelines of National Institutes of Health (NIH): Mild illness that show symptoms like fever, cough, nausea, vomiting, sore throat, loss of taste \& smell but don't show dyspnea or abnormal chest imaging. Moderate illness that show clinical or radiological lower respiratory disease with SpO2 \> 94% on room air.
Severe to critical COVID 19 patientsSclerostinAccording to COVID 19 treatment guidelines of National Institutes of Health (NIH): Severe illness that show SpO2 \< 94% on room air, (PaO2/FiO2) \<300 mm Hg \& lung infiltration \> 50% with respiratory rate \> 30 breath/min. Critical illness that show respiratory failure, septic shock or multiorgan failure.
Mild to moderate COVID 19 patientsparathyroid hormoneAccording to COVID 19 treatment guidelines of National Institutes of Health (NIH): Mild illness that show symptoms like fever, cough, nausea, vomiting, sore throat, loss of taste \& smell but don't show dyspnea or abnormal chest imaging. Moderate illness that show clinical or radiological lower respiratory disease with SpO2 \> 94% on room air.
Severe to critical COVID 19 patientsCalciumAccording to COVID 19 treatment guidelines of National Institutes of Health (NIH): Severe illness that show SpO2 \< 94% on room air, (PaO2/FiO2) \<300 mm Hg \& lung infiltration \> 50% with respiratory rate \> 30 breath/min. Critical illness that show respiratory failure, septic shock or multiorgan failure.
Normal male subjects (Control)Fibroblast growth factor 23-
Normal male subjects (Control)parathyroid hormone-
Primary Outcome Measures
NameTimeMethod
changes in FGF23 levels6 months

Expected higher FGF23 levels in COVID 19 patients compared to control

Changes in parathyroid hormone levels6 months

Expected lower levels in COVID 19 patients compared to control

Changes in serum calcium levels6 months

Expected lower serum calcium levels in COVID 19 patients compared to control

changes in sclerostin levels6 months

Expected higher sclerostin levels in COVID 19 patients compared to control

Secondary Outcome Measures
NameTimeMethod
Relation between FGF 23 and calcium levels6 months

A negative relation is expected

Relation between sclerostin and calcium levels6 months

A negative relation is expected

Trial Locations

Locations (1)

Aswan University hospital

🇪🇬

Aswan, Egypt

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