Impact of Intermittent Hypoxia on the Number and Function of Regular T Cell in Patients With Obstructive Sleep Apnea
试验速览
- 阶段
- 不适用
- 入组人数
- 80
- 试验地点
- 1
- 主要终点
- The number and function of regular T cells in peripheral venous blood
研究概览
简要总结
To compare the number and function of regular T cell of patients with obstructive sleep apnea (OSA) versus that of age-, gender- and BMI (body mass index)-matched controls
详细描述
Patients with obstructive sleep apnea (OSA) experience intermittent hypoxemia and arousal during sleep due to recurrent collapse of upper airway. Epidemiologic evidence showed they were at higher risk for autoimmune diseases. The regular T cell (Treg) has a crucial role in controlling autoimmunity. In this study, 40 OSA patients and 40 age-, gender- and BMI-matched controls will be recruited. Peripheral venous blood of the participants is drawn for numeration of CD4+CD25+FoxP3+ regular T cells by flow cytometry. As well, the CD4+CD25+ regular T cells are enriched by MACS and tested for their suppressive functions on proliferation assays. Th12+ CD4+ cells are also measured.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Prospective
入排标准
- 年龄范围
- 20 Years 至 60 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •OSA patients diagnosed with a polysomnography
- •Agree to participate the study and sign informed consent
排除标准
- •Do not agree to participate the study and sign informed consent
- •Has received any treatment or OSA
- •With major comorbidities (such as a diabetes, heart disease, lung diseases, renal diseases, autoimmune diseases, but hypertension excluded)
- •History of sarcoidosis and tuberculosis
- •On treatment of steroid and immunosuppressant
结局指标
主要结局
The number and function of regular T cells in peripheral venous blood
时间窗: 0-26 week
次要结局
- The number of Th12+ CD4+ cells(0-26 week)
研究者
vghtpe user
Chou, Kun-Ta
Taipei Veterans General Hospital, Taiwan