Blood and Muscle Response to Cheddar Cheese in Healthy Adults

Not Applicable

Completed

• Conditions: Healthy
• Interventions: Dietary Supplement: Cheddar Cheese; Dietary Supplement: Milk

• Registration Number: NCT04660877
• Lead Sponsor: University of Utah

Brief Summary

The goal of this project is to use a cross over design in young healthy male and female adults:

1. To determine the amino acid blood response to the acute ingestion of cheddar cheese in comparison to that of milk.

2. To determine the muscle mTORC1 response to the acute ingestion of cheddar cheese in comparison to that of milk.

Detailed Description

Each subject will take part in two metabolic studies. Each metabolic study was designed to test the acute blood and muscle response to an ingested amount of either cheddar cheese or milk. Approximately, one month after the first experiment (Metabolic Study #1), the participant will complete the second experiment (Metabolic Study #2) which was exact in design and at the same time of day as the first study but the participant ingested the alternate food product. The morning of the metabolic studies, the participant will arrive at the clinical research center after a 10h fast. A catheter will be placed in the participants' arm for blood sampling. Next, the participant will undergo a fasted thigh skeletal muscle biopsy. Following the muscle biopsy, the participant will consume either cheddar cheese (65g) or milk (370 mL) each amounting to 20g of protein. Subsequent muscle biopsies will occur at 60 and 180min after product ingestion. Blood sampling will occur in the fasted state and periodically after ingestion of the products (up to 300min).

Study Timeline

• Study Start: 2018-06-28
• Primary Completion: 2019-08-20
• Study Completion: 2019-08-20
• Study First Submitted: 2020-12-01
• Study First Submitted QC: 2020-12-08
• Study First Posted: 2020-12-09
• Status Verified: 2022-06
• Last Update Submitted: 2022-06-17
• Last Update Posted: 2022-06-22

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 42

Inclusion Criteria

• Age between 18-35y

Exclusion Criteria

1. History of cardiovascular disease
2. History of endocrine or metabolic disease (e.g., hypo/hyperthyroidism, diabetes)
3. History of kidney disease or failure
4. History of liver disease
5. History of respiratory disease (acute upper respiratory infection, chronic lung disease)
6. History of stroke with motor disability
7. Use of anticoagulant therapy (e.g., Coumadin, heparin) including aspirin and fish oils within 7d of the first biopsy experiment
8. Elevated systolic blood pressure >150 or a diastolic blood pressure > 100
9. Smoking
10. Recent anabolic or corticosteroids use (within 12 weeks)
11. Pregnancy as determined by a pregnancy test
12. self report of lactose intolerance
13. Any other medical condition or event considered exclusionary by the PI and faculty physician

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
Cheddar chese	Cheddar Cheese	Once enrolled, participants will either ingest 20 grams of protein from 1) cheese or 2) milk for the first Metabolic Study and then the other product when they return after the washout period (\~1 month) for Metabolic Study #2.
Milk	Milk	Once enrolled, participants will ingest 20 grams of protein from 1) cheese or 2) milk for the first Metabolic Study and then the other product when they return after the washout period (\~1 month) for Metabolic Study #2.

Primary Outcome Measures

Name	Time	Method
Response to cheese or milk ingestion	Area under the curve 0-300min after ingestion	blood amino acids area under the curve in response to acute stimulation of cheese or milk consumption

Secondary Outcome Measures

Name	Time	Method
Response to cheese or milk ingestion	Change between 60min after ingestion and fasted response	muscle mTORC1 change in response to acute stimulation of cheese or milk

Trial Locations

Locations (1)

The University of Utah; 🇺🇸 Salt Lake City, Utah, United States