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Chronic Periodontitis and Polycystic Ovary Syndrome

Completed
Conditions
Polycystic Ovary Syndrome
Chronic Periodontitis
Interventions
Other: no intervention provided
Registration Number
NCT02954120
Lead Sponsor
Bezmialem Vakif University
Brief Summary

Background: The aim of this study was to investigate the levels of malondialdehyde (MDA), total antioxidant status (TAS), 8-hydroxy-2´- deoxyguanosine (8-OHdG) in blood serum and saliva samples in female patients with chronic periodontitis (CP) and to compared healthy individuals.

Materials and Methods: A total of 88 women were into four groups each which were consisted of twenty-two subjects. Groups were designed as periodontally and systemically healthy women (PCOS-CP-); periodontally healthy women with polycystic ovary syndrome (PCOS) (PCOS+CP-), systemically healthy women with CP (PCOS-,CP+) and women with PCOS and CP (PCOS+CP+). Serum and salivary samples were obtained, clinical periodontal parameters were recorded. MDA, TAS, 8-OHdG levels were measured as biochemically.

Detailed Description

RESULTS:

Salivary 8-OHdG levels in the PCOSCP and CP groups were statistically higher than those in both the PCOSPH and the PH groups (P \< 0.05). There was no statistical difference between the PCOSCP, CP, and PCOSPH groups with regard to salivary MDA and TAS levels (P \> 0.05). Highest serum 8-OHdG and MDA levels and lowest serum TAS levels were seen in the PCOSCP group (P \< 0.05). Serum 8-OHdG and MDA levels in the PCOSPH group were higher than those in both systemically healthy groups (PH and CP) (P \< 0.05). Salivary TAS levels were highest (P \< 0.05) in the PH group. There was no statistical difference between the CP and PCOSPH groups, but serum TAS levels were lower than those in the PH group (P \< 0.05).

CONCLUSIONS:

CP, which led to an increase in serum and salivary 8-OHdG and MDA levels and a decrease in serum TAS levels in patients with PCOS, contributed to increased OS. This effect was more prominent in serum levels than in salivary levels.

Recruitment & Eligibility

Status
COMPLETED
Sex
Female
Target Recruitment
88
Inclusion Criteria
  • Newly diagnosed non-obese PCOS patients
  • Never smokers
  • Had no history of systemic disease
  • BMI<25 kg/m2
  • Participants had ≥20 teeth present.
Exclusion Criteria
  • Pregnancy
  • Lactation
  • Hemoglobin A1c (HbA1c) ≥ 6.5%
  • 2-h oral glucose tolerance test (OGTT-2h) ≥200
  • Cushing syndrome, non-classic congenital adrenal hyperplasia, hyperprolactinemia, thyroid dysfunction, and androgen-secreting tumors
  • Any drug use within the past 6 months
  • Periodontal treatment within the past 6 months

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Arm && Interventions
GroupInterventionDescription
(PCOS-CP+)no intervention providedsystemically healthy participants with CP
(PCOS-CP-)no intervention providedsystemically and periodontally healthy participants
(PCOS+CP-)no intervention providedPCOS participants with periodontally healthy
(PCOS+CP+)no intervention providedPCOS participants with CP
Primary Outcome Measures
NameTimeMethod
Gingival Index (GI)appoximately 2 years

Löe-Silness Periodontal Index

Bleeding on probing (BOP)appoximately 2 years

Gİngival Bleeding Index

Probing pocket depth (PD))appoximately 2 years

distance between free gingival margin and periodontal pocket base by usin periodontal probe

8-OHdG levels (nanogram per mililiter(ng/mL))appoximately 2 years

by ELISA test

Plaque Index (PI)appoximately 2 years

Silnes-Löe Periodondal İndex

Clinical attachment level (CAL)Patients were seen one time

by measuring the distance between the cementoenamel junction and the periodontal pocket base

TAS levels (millimolar per liter (mmol /L))appoximately 2 years

by ELISA test

MDA levels (micromole per liter (µmol/L))appoximately 2 years

by ELISA test

Secondary Outcome Measures
NameTimeMethod
numberer of individuals with polycystic ovary syndromeappoximately 2 years

individuals in the group

number of individuals with chronic periodontitisappoximately 2 years

individuals in the group

Trial Locations

Locations (1)

Department of Periodontics, Faculty of Dentistry, Bezmialem Vakif University

🇹🇷

İstanbul, Fatih, Turkey

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