Cohort of Twin Pregnancy and the Offspring
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Twin Pregnancy
- Sponsor
- Fudan University
- Enrollment
- 1000
- Locations
- 1
- Primary Endpoint
- Protein profiles in placentas of twin pregnancy measured by tandem mass tags quantification.
- Status
- Recruiting
- Last Updated
- last year
Overview
Brief Summary
Twin pregnancy increases the risk of maternal and fetal complications, which include gestational hypertension, premature labor, twin-to-twin transfusion syndrome, intrauterine growth restriction, anemia, amniotic fluid abnormalities. Comprehensively understanding the molecular mechanisms of the disease and identification of markers contribute to development of novel therapeutic approaches. In addition, the twin pregnancy, especially the monochorionic, is an essential model of "experiments of nature". This model can be applied to distinguish the epigenetic differences of twins in utero and after birth in the same genomic context. The aim of the study is to constitute a prospective cohort of twin pregnancies and the offspring.
Detailed Description
Data are collected regarding the pregnancy data of the mother, and mental, physical and sociodemographic information of the offspring. The periods span from diagnosis of twins to 3 years old of the offspring. Biological samples would be collected including maternal blood, placental tissues, cord blood, urine, and stool from the pregnant women or the offspring at the specific time. The total estimated duration of the study is 6 years, of which the first 2.5 years will correspond to the recruitment period of the participants.
Investigators
Yu Xiong
Clinical Professor
Fudan University
Eligibility Criteria
Inclusion Criteria
- •Maternal age \>18 years old.
- •Twin pregnancy.
Exclusion Criteria
- •Fetal chromosomal or congenital abnormalities.
- •Stillbirth.
Outcomes
Primary Outcomes
Protein profiles in placentas of twin pregnancy measured by tandem mass tags quantification.
Time Frame: Immediately after delivery
Levels of proteins in placentas of twin pregnancy.
Lipid profiles in placentas of twin pregnancy measured by lipidomic analysis
Time Frame: Immediately after delivery
Lipid profiles in placentas of twin pregnancy.
Global DNA methylation in placentas of twin pregnancy measured by whole-genome bisulfite sequencing
Time Frame: Immediately after delivery
Global DNA methylation levels in placentas of twin pregnancy.
Metabolite profiles in placentas of twin pregnancy measured by metabolomics
Time Frame: Immediately after delivery
Metabolite profiles in placentas of twin pregnancy.
Predictive lipid markers of maternal and fetal complications in maternal blood measured by lipidomic analysis
Time Frame: From confirmation of pregnancy to 42 days postpartum
Levels of specific lipids in maternal blood.
Post-translation modification of proteins in placentas of twin pregnancy measured by Integrative use of phosphoproteomics, ubiquitylproteomics and acetylproteomics.
Time Frame: Immediately after delivery
Post-translational levels of proteins in placentas of twin pregnancy.
The incidence of maternal and fetal complications
Time Frame: From confirmation of pregnancy to 42 days postpartum
The incidence of preeclampsia, gestational diabetes mellitus, premature delivery, postpartum hemorrhage, twin to twin transfusion syndrome, selective intrauterine growth restriction.
RNA profiles in placentas of twin pregnancy measured by RNA-sequencing
Time Frame: Immediately after delivery
Levels of placental RNA expression.
Predictive protein markers of maternal and fetal complications in maternal blood measured by tandem mass tags quantification
Time Frame: From confirmation of pregnancy to 42 days postpartum
Levels of specific proteins in maternal blood.
Predictive RNA markers of maternal and fetal complications in maternal blood measured by RNA-sequencing
Time Frame: From confirmation of pregnancy to 42 days postpartum
Levels of specific RNAs in maternal blood.
Predictive metabolite markers of maternal and fetal complications in maternal blood measured by metabolomics
Time Frame: From confirmation of pregnancy to 42 days postpartum
Levels of specific metabolites in maternal blood.
Secondary Outcomes
- The incidence of long term maternal complications(Three years after delivery)
- The level of twin Neurodevelopment assessed by Gesell Developmental Schedules(Age at 3 years of the offspring)