An Intervention Study of Sustainable Food Habits That Promotes Health and Environmental Aspects
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 39
- 试验地点
- 2
- 主要终点
- Changes in concentration of pesticide residues in urine
研究概览
简要总结
The aim of this study is to investigate changes in nutrient intake, the human gut microbiota and pesticide excretion in urine when shifting from conventional food habits to sustainable food habits.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Other
- 盲法
- Single (Participant)
入排标准
- 年龄范围
- 20 Years 至 55 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Living in Västerås
- •Carnivores
排除标准
- 未提供
结局指标
主要结局
Changes in concentration of pesticide residues in urine
时间窗: Baseline, after 4 weeks, after 8 weeks
The investigators will assess changes in concentration of four pesticide residues (Mepiquat, Chlormequat, Propamocarb and Ethylene thiourea) excreted in urine. Samples will be collected at three occasions; baseline (reference sample), midtime (after 4 weeks) and at the end (after 8 weeks). The concentration of pesticide residues in urine samples will be determined in normal or reversed phase configuration on a high performance liquid chromatograph coupled to a triple quadrupole mass spectrometer (HPLC-MS/MS) system with electrospray ionization interface (ESI) run in negative or positive mode.
Changes in composition of the microbiota
时间窗: Baseline, after 4 weeks, after 8 weeks
The investigators will assess changes over time in the presence of all gut bacteria species in the microbiome of the participants based on DNA sequencing from fecal samples. Samples will be collected at three occasions; baseline (reference sample), midtime (after 4 weeks) and at the end (after 8 weeks). From frozen samples, all DNA will be extracted and full shotgun metagenomic sequencing performed using next generation sequencing technologies (Illumina and BGISEQ). A total of 120 samples (3\*40 participants) will be sequenced to a depth of at least 3 giga base pairs (Gbp) each.
次要结局
- Changes in intake of zinc(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of selenium(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of fat(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of thiamin(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of riboflavin(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of carbohydrates(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of vitamin E(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of niacin(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of vitamin B6(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of iodine(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of energy(After 8 weeks)
- Changes in intake of protein(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of dietary fiber(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of vitamin A(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of vitamin D(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of folate(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of vitamin C(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of iron(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of calcium(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of phosphorus(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of vitamin B12(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of potassium(Baseline, after 4 weeks, after 8 weeks)
- Changes in intake of copper(Baseline, after 4 weeks, after 8 weeks)
- Assessment of the participants likeability of the diets using a questionnaire(After 8 weeks)