Adipose Tissue Blood Flow in Aging Humans
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 发起方
- Mayo Clinic
- 入组人数
- 24
- 试验地点
- 1
- 主要终点
- Adipose tissue blood flow
研究概览
简要总结
The goal of this basic science clinical trial is to understand whether adipose tissue blood flow changes between younger and older healthy adults.
The main question[s] it aims to answer are:
- Is basal and nutrient responsive adipose tissue blood flow (ATBF) different between younger and older adults
- What molecular and systemic signatures related to adipose tissue blood flow are altered between these two groups.
Participants will undergo measurements of adipose tissue blood flow using the xenon washout technique, undergo 2 abdominal subcutaneous adipose tissue biopsies, and drink a sugary drink.
详细描述
On Visit 1, volunteers will provide written informed consent (and urine pregnancy test for women of childbearing age), complete a Dual x-ray absorptiometry scan, baseline labs (comprehensive metabolic panel) and a cardiopulmonary exercise test. Participants will arrive fasted at 08:00, and urine pregnancy test for women of childbearing age administered and an intravenous catheter will be placed. Following 30min of supine rest, baseline venous blood samples will be collected. ATBF will be assessed on participants' left abdomen first by measurement of blood flow using 133Xenon washout method. Immediately thereafter, an abdominal adipose tissue biopsy of the left side will be performed but ≥6 cm distant from location of 133Xenon injections. Subjects will them consume 75 gm glucose; because ATBF peaks 30-60 minutes following ingestion of glucose, ATBF measures will start 30 minutes after ingestion on the contralateral side followed immediately by plasma measures and another adipose tissue biopsy. During ATBF measurements, brachial blood pressure will be assessed in two-minute intervals.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 80 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Within the ages of 18-35 years or 65-80 years at time of study visit
- •BMI between 20-25.0 kg/m2.
排除标准
- •Presence of diagnosed cardiometabolic diseases (e.g., Type 2 diabetes, hypertension, Heart Failure)
- •Taking prescription anticoagulants
- •Taking prescriptions or supplements that effect adipose tissue metabolism (i.e., statins, Thiazolidinediones, niacin, atypical antipsychotics, or fish oil)
- •Pregnant or nursing
- •Inability to provide written informed consent
研究组 & 干预措施
Older Adults
干预措施: 75 gm oral glucose load (Other)
Younger Adults
干预措施: 75 gm oral glucose load (Other)
结局指标
主要结局
Adipose tissue blood flow
时间窗: Baseline, pre-intervention fasting and immediately following 75 gm oral glucose load
blood flow will be measured in the upper body subcutaneous adipose tissue using 133 Xenon washout and reported as blood flow in ml/min/100g adipose tissue.
次要结局
- Genomic alterations(Baseline, pre-intervention fasting and immediately following 75 gm oral glucose load)
研究者
Kelli A. Lytle
Principal Investigator
Mayo Clinic