Effects of IVF culture conditions on the embryonic genome, methylome and transcriptome: a study to evaluate the safety and quality of different culture systems

Conditions: subfertiliteit
subfertility

Registration Number: NL-OMON48079

Lead Sponsor: Medisch Universitair Ziekenhuis Maastricht

Brief Summary: Not available

Detailed Description: Not available

Recruitment & Eligibility

Status: Pending

Sex: Not specified

Target Recruitment: 219

Inclusion Criteria

- MUMC: embryos from couples who took part in the MEDIUMtrial-0 or the
MEDIUMtrial-1
- UMCG: embryos from couples who underwent an IVF treatment between January
2012 and December 2013.

Exclusion Criteria

- embryo's that do not survive the thawing procedure

Study & Design

Study Type: Interventional

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>a) To evaluate the effect of 4 (previously) widely used IVF culture media<br /><br>(Sydney IVF Medium, G3, G5 and HTF) on the genome, epigenome (mainly methylome<br /><br>and chromatin accessibility) and transcriptome of human pre-implantation<br /><br>embryos. These culture media have been associated with differences in early<br /><br>life outcomes.<br /><br>b) To evaluate the effect of preimplantation human embryo culture at different<br /><br>oxygen concentrations (5% and 20% oxygen), on the genome, epigenome (mainly<br /><br>methylome and chromatin accessibility) and transcriptome of pre-implantation<br /><br>human embryos. Both concentrations have been widely used in human ART.</p><br>

Secondary Outcome Measures

Name	Time	Method
<p>c) To establish whether there are links between the epigenome (mainly methylome<br /><br>and chromatin accessibility), genome and transcriptome that could explain high<br /><br>levels of CIN seen in human IVF embryos.<br /><br>d) To develop in silico methods for lineage tracing that can be used to<br /><br>determine the fate of chromosomally abnormal cells during embryo development.</p><br>