Development of an assay for detection of Circulating Endothelial Cell (CEC) and Circulating Endothelial Progenitor cell (CEP) by fluorescence-activated cell sorting (FACS) in cancer patients and healthy individuals.
- Conditions
- Cancermalignancy10027655
- Registration Number
- NL-OMON40594
- Lead Sponsor
- Antoni van Leeuwenhoek Ziekenhuis
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Recruiting
- Sex
- Not specified
- Target Recruitment
- 70
bInclusion criteria cancer patients:
1. Patients with histological or cytological proof of metastatic cancer.
2. Age * 18 years
3. Able and willing to give written informed consent
4. Able and willing to undergo blood sampling ;Inclusion criteria healthy volunteers:
1. Age * 18 years
2. Able and willing to give written informed consent
3. Able and willing to undergo blood sampling
Exclusion criteria cancer patients:
1. Patients with known alcoholism, drug addiction, psychotic disorders in the history and/or other reasons, for which they are not amenable for adequate follow up.
2. Uncontrolled infectious disease or known HIV-1 or HIV-2 type patients ;Exclusion criteria healthy volunteers:
1. Patients with known alcoholism, drug addiction, psychotic disorders in the history and/or other reasons, for which they are not amenable for adequate follow up.
2. Uncontrolled infectious disease or known HIV-1 or HIV-2 type patients
3. Prior history of malignancy, renal disease, cardiovascular disease.
Study & Design
- Study Type
- Observational invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>A reproducible method for enumeration of CECs and CEPs in peripheral blood<br /><br>using FACS</p><br>
- Secondary Outcome Measures
Name Time Method <p>Gain further insight in the origin of CECs and CEPs.</p><br>