Effect of Passive Smoking on Oxidative Stress and Dental Caries in Children

Completed

• Conditions: Passive Smoking; Oxidative Stress; Dental Caries
• Interventions: Diagnostic Test: taking saliva samples to determine oxidative stress markers TAS and TOS

• Registration Number: NCT04298996
• Lead Sponsor: Merve Erkmen Almaz

Brief Summary

The purpose of the present study was to determine the effect of passive smoking on oxidative stress and dental caries in children.A total of 180 children were included to the study as follows; 90 children exposed to passive smoking, and 90 children in the control group (unexposed controls). Also demographic data were recorded (age, gender, parental education levels, child's tooth-brushing habit and child's daily dietary sugar exposure, family income). Dental examination of children were performed and caries prevalence of the patients were recorded. Unstimulated saliva samples were collected from children. Saliva 'cotinine levels' and oxidative stress markers 'total antioxidant status' (TAS), and' total oxidant status' (TOS) were evaluated by using Enzyme-linked immunosorbent assay (ELISA). The obtained data parameters of the two groups were evaluated and comparison was performed.

Detailed Description

Not available

Study Timeline

• Study Start: 2016-11-16
• Primary Completion: 2018-04-15
• Study Completion: 2018-08-15
• Status Verified: 2020-03
• Study First Submitted: 2020-03-04
• Study First Submitted QC: 2020-03-04
• Last Update Submitted: 2020-03-05
• Study First Posted: 2020-03-06
• Last Update Posted: 2020-03-09

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 180

Inclusion Criteria

-Systemically healthy children

Exclusion Criteria

• Uncooperative children
• The presence of orthodontic/intraoral appliance
• Any disease or condition known to affect oxidative stress

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
control group	taking saliva samples to determine oxidative stress markers TAS and TOS	examining for caries prevalence and taking saliva samples to determine oxidative stress markers TAS and TOS
study group (passive smoking children)	taking saliva samples to determine oxidative stress markers TAS and TOS	examining for caries prevalence and taking saliva samples to determine oxidative stress markers TAS and TOS

Primary Outcome Measures

Name	Time	Method
determining caries prevalence	1 day (once for dental examination)	Children were examined to determine caries prevalence.
determining oxidative stress marker in saliva: TOS	1 day (once after the dental examination)	Unstimulated saliva samples were collected from children.
determining oxidative stress marker in saliva: TAS	1 day (once after the dental examination)]	Unstimulated saliva samples were collected from children.