Salivary TAS, TOS, LL-37 and Dental Status in Passive Smoking Children

Completed

• Conditions: Oxidative Stress; Passive Smoking; Dental Caries; Children
• Interventions: Diagnostic Test: taking saliva samples to determine TAS, TOS and LL-37

• Registration Number: NCT04292548
• Lead Sponsor: Merve Erkmen Almaz

Brief Summary

The aim of the present study was to determine the effect of passive smoking on the levels of salivary TAS, TOS, LL-37 and dental status in children.In the study the parents of children were asked to fill out a complete survey about smoking habits. After filling out the questionnaire, according to the survey results, a total of 180 children were included to the study as follows; 90 children exposed to passive smoking, and 90 children in the control group (unexposed controls). Also demographic data were recorded (age, gender, parental education levels, child's tooth-brushing habit and child's daily dietary sugar exposure, family income). Dental examination of children were performed and caries prevalance of the patients were recorded. Unstimulated saliva samples were collected from children. Saliva 'cotinine levels' which are expected to increase in passive smoking group; 'antimicrobial peptide LL-37' and oxidative stress markers 'total antioxidant status' (TAS), and' total oxidant status' (TOS) were evaluated by using Enzyme-linked immunosorbent assay (ELISA). The obtained data parameters of the two groups were evaluated and comparison was performed.

Detailed Description

Not available

Study Timeline

• Study Start: 2016-11-16
• Primary Completion: 2018-04-15
• Study Completion: 2018-08-15
• Status Verified: 2020-02
• Study First Submitted: 2020-02-28
• Study First Submitted QC: 2020-03-02
• Last Update Submitted: 2020-03-02
• Study First Posted: 2020-03-03
• Last Update Posted: 2020-03-03

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 180

Inclusion Criteria

-Systemically healthy children

Exclusion Criteria

• The presence of orthodontic / intraoral appliance
• Any disease or condition known to affect defense system
• Uncooperative children

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
control group	taking saliva samples to determine TAS, TOS and LL-37	-
study group (passive smoking children)	taking saliva samples to determine TAS, TOS and LL-37	-

Primary Outcome Measures

Name	Time	Method
salivary LL-37 level	1 day (once after the dental examination)	Unstimulated saliva samples were collected from children.
salivary oxidative stress values: TAS level	1 day (once after the dental examination)	Unstimulated saliva samples were collected from children.
salivary oxidative stress values: TOS level	1 day (once after the dental examination)	Unstimulated saliva samples were collected from children.
salivary cotinine levels	1 day (once after the dental examination)	Unstimulated saliva samples were collected from children for describing passive smoking children.