Effect of Ca2+ Ionophore on Embryonic Development and Clinical Outcome in Cases With Previous Fertilization Arrest
Not Applicable
- Conditions
- Fertilization Arrest
- Interventions
- Other: Ca2+ ionophore
- Registration Number
- NCT02683031
- Lead Sponsor
- TopLab Company for ART Laboratories Consultation and Training
- Brief Summary
We aimed to investigate the efficiency of chemical oocyte activation using readymade Ca2+ ionophore on cases with previously cancelled cases due to fertilization arrest at pronuclear stage i.e: cleavage failure to find out if there is any improvement in embryonic developmental stages.
- Detailed Description
Many studies reported that Ca2+ ionophore has a positive effect on embryo development in cases with complete or partial fertilization failure. However, only one preliminary report suggested a beneficial effect of Ca2+ ionophore activation in four cases with fertilization arrest.
Recruitment & Eligibility
- Status
- UNKNOWN
- Sex
- Female
- Target Recruitment
- 50
Inclusion Criteria
- Patients with previous history of complete fertilization arrest.
- ovarian stimulation with the GnRH agonist and recombinant FSH in a long protocol.
Exclusion Criteria
- Female age >40 years
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Arm && Interventions
Group Intervention Description With Ca2+ ionophore Ca2+ ionophore Patients with previous incomplete ICSI cycle due to fertilization arrest were counseled to underwent a subsequent ICSI cycle combined with artificial oocyte activation using Ca2+ ionophore.
- Primary Outcome Measures
Name Time Method Blastocyst formation rate 5 to 7days post oocyte retrival Defined as the total number of blastocyst / total number of cleaved Embryos
- Secondary Outcome Measures
Name Time Method
Trial Locations
- Locations (1)
Ahmed el-Damen
🇪🇬Cairo, Egypt