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YQ23 Study in Patients With Critical Limb Ischaemia

Phase 1
Terminated
Conditions
Critical Limb Ischemia
Interventions
Biological: Matching placebo
Biological: YQ23
Registration Number
NCT04792008
Lead Sponsor
New Beta Innovation Limited
Brief Summary

This is an early phase study to assess how safe and tolerable is the new study drug YQ23 and to compare the effectiveness of YQ23 against normal saline to treat critical limb ischaemia. The study also aims to understand how it affects the body and an optional substudy to assess how the human body takes up, breaks down, and clears the study drug.

Eligible patients will be randomised to have a 2:1 chance to receive a single, intravenous, fixed dose of YQ23 or normal saline. Neither the patient nor the study site will know which treatment has been given.

On the day of YQ23 administration, patients will be asked to stay in the study site for 3 days for safety observation. After discharge, they will be required to visit the study clinic for 3 times in a year to continue safety monitoring and assessment of treatment effect.

Each subject's participation will be about 13 months after signing the informed consent.

Detailed Description

This is a Phase 1b/2a, randomised, double-blind, placebo-controlled study to evaluate the safety, tolerability, and efficacy of an investigational product, YQ23, in patients with Critical Limb Ischaemia (CLI) during a follow-up period of 12 months.

Fifty-one patients are planned for enrolment. The study consists of a screening period (up to 28 days), a double-blind treatment period, and a follow-up (12 months). Prior to randomisation, patients diagnosed with CLI will be stratified into:

* Group of patients in whom revascularisation is not planned

* Group of patients with planned revascularisation

Within each group, patients will be randomised in a 2:1 ratio to receive single intravenous infusion of YQ23 120 mg/kg and normal saline, respectively at the study site.

On the day of YQ23 administration, the patient will be admitted to the study site on Day 1 and will be discharged on Day 3. The total duration of participation in the study for each patient is approximately 13 months. Data on the study endpoints will be collected from baseline (pre-dose on Day 1), Day 3, Month 1, 6 and up to 12 months after study treatment infusion.

Recruitment & Eligibility

Status
TERMINATED
Sex
All
Target Recruitment
5
Inclusion Criteria
  • Diagnosis of CLI (Rutherford Classification stage 4, 5 or 6) including at least one of the following:

    1. resting ankle systolic pressure (either dorsalis pedis or posterior tibial artery) <=70 mmHg in affected limb
    2. resting toe systolic pressure <=50 mmHg in affected limb
    3. TcPO2 <=30 mmHg
  • One of the following clinical presentations:

    1. pain at rest
    2. ischaemic ulcer, and/or focal gangrene for at least 2 weeks
  • Diagnosis of severe lower extremity peripheral artery occlusive disease as evidenced by either:

    1. Documented significant stenosis (>=75%) of >=1 of the following arteries: superficial femoral, popliteal, and infra-popliteal arteries, as assessed by imaging test, or
    2. ABI <=0.80 or TBI <=0.60 of the index leg (in the event of non-compressible ankle arteries) for patients without a prior history of limb revascularization on the index leg, or an ABI <=0.85 or TBI <=0.65 of the index leg (in the event of non-compressible ankle arteries) for patients with a prior history of limb revascularization on the index leg.
  • Contraceptive use

    1. Male patients and their female spouses/partners who are of childbearing potential must agree to use a high effective contraception consisting of two forms of birth control detailed in the protocol during the treatment period and for at least 6 days after the dose of the study treatment and refrain from donating sperm during this period
    2. A female patient is eligible if she is not pregnant, not breastfeeding, and at least on of the following conditions applies: (i) not a woman of childbearing potential (WOCBP), (ii) A WOCBP who agrees to follow the contraceptive guidance in the protocol during the treatment period and for at least 6 days after the dose of study treatment and refrain from donating ova during this period
  • Patient is capable of giving signed informed consent which includes compliance with the requirements and restrictions listed in the informed consent and the protocol

Exclusion Criteria
  • Patients who have undergone successful revascularisation on the index leg within 4 weeks of the qualifying event
  • Patients with estimated life expectancy <12 months
  • Acute limb ischaemia due to thromboembolism within 2 weeks of the qualifying event
  • Recent myocardial infarction within 30 calendar days prior to signing informed consent
  • Recent stroke within 30 calendar days prior to signing informed consent
  • Known history of severe congestive heart failure as determined through review of medical history
  • Haemoglobin <8 g/dL, albumin <3 g/dL or other clinically significant abnormalities in the laboratory tests at screening
  • Unwilling to complete follow-up evaluation
  • Uncontrolled arterial hypertension with systolic blood pressure (SBP) >180 mmHg and/or diastolic blood pressure (DBP) >100 mmHg at screening
  • Patients with history of long QT syndrome or whose QTc (calculated according to Bazett formula QTc = QT/ √RR) >470 ms at screening
  • Patients with severe left ventricular dysfunction of <40% at screening
  • Patients with clinically significant abnormalities in ECG parameters (other than QTc) or in the physical examination at screening that might comprise patient safety
  • History of coagulopathy
  • Patients having significant renal impairment with estimated glomerular filtration rate (eGFR) <30 mL/min/1.73 m2 as determined by the 4-variable Modification of Diet in Renal Disease (MDRD) equation at Screening, except those patients who are on continuous ambulatory peritoneal dialysis(CAPD) or hemodialysis (HD)
  • Significant liver impairment (abnormal Liver Function Tests >3 x upper limit of normal)
  • Patients with active Hepatitis B infection (HBsAg positive and HBeAg positive) at screening
  • Patient with a positive test for Hepatitis C virus antibody (anti-HCV) at screening
  • Patient with known history of infection with human immunodeficiency virus (HIV) or any other active or chronic infection
  • Patients with evidence of uncontrolled hypo- or hyperthyroidism
  • History active malignancy (as determined through review of medical history), excluding local skin cancer (basal or squamous cell carcinoma)
  • Patients unable to provide informed consent
  • Known allergy to bovine products
  • Receipt of bovine haemoglobin-based oxygen carrier (HBOC) or other HBOC in the past
  • Use of an investigational drug or treatment within 12 months prior to signing informed consent; concurrent participation in any other investigational protocol
  • Pregnancy and breastfeeding, as well as unwillingness to use effective methods contraception for women of childbearing potential

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Placebo Single doseMatching placeboOne-third of randomized patients will receive matching placebo
YQ23 Single doseYQ23Two-third of randomized patients will receive YQ23 as active treatment
Primary Outcome Measures
NameTimeMethod
Safety and tolerability of single IV dose of YQ23 - adverse and serious adverse eventsFrom the time of signing informed consent through study completion, a duration of 13 months.

Incidence of adverse events and serious adverse events

Safety and tolerability of single IV dose of YQ23 - 12 lead electrocardiogram (ECG)From the time of signing informed consent through study completion, a duration of 13 months.

Number of participants with a change in 12-lead ECG measurements which is of clinical significance

Safety and tolerability of single IV dose of YQ23 - vital signsFrom the time of signing informed consent through study completion, a duration of 13 months.

Number of participants with a change in vital signs of blood pressure, pulse rate, respiratory rate or oral temperature which is of clinical significance

Safety and tolerability of single IV dose of YQ23 - abnormal laboratory valuesFrom the time of signing informed consent through study completion, a duration of 13 months.

Number of participants with a change in laboratory values of haematology, chemistry or urinalysis which is of clinical significance

Safety and tolerability of single IV dose of YQ23 - physical examinationsFrom the time of signing informed consent through study completion, a duration of 13 months.

Number of participants with a change in physical examination findings which is of clinical significance

Safety and tolerability of single IV dose of YQ23 - major adverse limb events (MALE)From pre-dose to Month 1

The incidence of MALE of interest. MALE include amputation (transtibial or above) or any major vascular intervention in the index limb

Secondary Outcome Measures
NameTimeMethod
Efficacy of single dose YQ23 as compared to placebo - wound healing 100%From pre-dose, Month 1, 6 and 12

Number of participants to achieve 100% epithelialization

Efficacy of single dose YQ23 as compared to placebo - transcutaneous oxygen pressureFrom pre-dose, 2 hours post-dose, Month 1, 6 and 12

Change in transcutaneous oxygen pressure (TcPO2)

Efficacy of single dose YQ23 as compared to placebo - amputation free survivalFrom pre-dose to Month 12

Incidence rate of all amputations

Efficacy of single dose YQ23 as compared to placebo - wound healing in sizeFrom pre-dose, Month 1, 6 and 12

Change in size as measured by length x width x depth in mm

Efficacy of single dose YQ23 as compared to placebo - quality of life scoreFrom pre-dose, Month 1, 6 and 12

Change in EuroQol -5 Dimension (EQ-5D) questionnaire

Efficacy of single dose YQ23 as compared to placebo - toe brachial index (TBI)From pre-dose, Month 1, 6 and 12

Change in TBI, a ratio of systolic blood pressure at the toe to that in the arm

An optional substudy to assess the pharmacokinetics of YQ23 - terminal elimination half-life (t1/2)From pre-dose, immediately after end of infusion, 0.25, 0.5, 1, 2, 6, 18 (+/-6) and 48 hours post end of infusion

Plasma level of YQ23 will be serially evaluated following dosing of the study drug to determine the t1/2

Efficacy of single dose YQ23 as compared to placebo - all cause mortalityFrom the time of signing informed consent to Month 12

Incidence rate of all deaths

Efficacy of single dose YQ23 as compared to placebo - MALE of interestAt month 6 and 12

Incidence of MALE of interest

Efficacy of single dose YQ23 as compared to placebo - Rutherford classificationFrom pre-dose, Month 1, 6 and 12

Change in Rutherford classification

Efficacy of single dose YQ23 as compared to placebo - visual analogue pain scaleFrom pre-dose, Month 1, 6 and 12

Change in visual analogue pain scale in a one to ten scale - one reported as no pain while 10 as the most intense pain

An optional substudy to assess the pharmacokinetics of YQ23 - maximum observed concentration (Cmax)From pre-dose, immediately after end of infusion, 0.25, 0.5, 1, 2, 6, 18 (+/-6) and 48 hours post end of infusion

Plasma level of YQ23 will be serially evaluated following dosing of the study drug to determine the Cmax

Efficacy of single dose YQ23 as compared to placebo - wound healing by Wound, Ischemia, and Foot Infection (WIFI) classification systemFrom pre-dose, Month 1, 6 and 12

Change in the grading under the WIFI classification system

An optional substudy to assess the pharmacokinetics of YQ23 - time to CmaxFrom pre-dose, immediately after end of infusion, 0.25, 0.5, 1, 2, 6, 18 (+/-6) and 48 hours post end of infusion

Plasma level of YQ23 will be serially evaluated following dosing of the study drug to determine the time corresponding to occurrence of the Cmax (Tmax)

An optional substudy to assess the pharmacokinetics of YQ23 - total clearance (CL)From pre-dose, immediately after end of infusion, 0.25, 0.5, 1, 2, 6, 18 (+/- 6) and 48 hours post end of infusion

Plasma level of YQ23 will be serially evaluated following dosing of the study drug to determine the CL

Efficacy of single dose YQ23 as compared to placebo - wound healing by transparent filmFrom pre-dose, Month 1, 6 and 12

Change in size as measured by the number of grids in a wound tracing film

Efficacy of single dose YQ23 as compared to placebo - ankle brachial index (ABI)From pre-dose, Month 1, 6 and 12

Change in ABI, a ratio of systolic blood pressure at the ankle to that in the arm

An optional substudy to assess the pharmacokinetics of YQ23 - Area under the curve (0 to infinity)From pre-dose, immediately after end of infusion, 0.25, 0.5, 1, 2, 6, 18 (+/-6) and 48 hours post end of infusion

Plasma level of YQ23 will be serially evaluated following dosing of the study drug to determine the area under the plasma concentration-time curve (AUC) - AUC from time 0 extrapolated to infinity (AUCinf)

An optional substudy to assess the pharmacokinetics of YQ23 - Area under the curve (0 to last)From pre-dose, immediately after end of infusion, 0.25, 0.5, 1, 2, 6, 18 (+/-6) and 48 hours post end of infusion

Plasma level of YQ23 will be serially evaluated following dosing of the study drug to determine the area under the plasma concentration-time curve (AUC) - AUC from time 0 to the last measurable time point (AUClast)

An optional substudy to assess the pharmacokinetics of YQ23 - volume of distribution during terminal phase (Vz)From pre-dose, immediately after end of infusion, 0.25, 0.5, 1, 2, 6, 18 (+/- 6) and 48 hours post end of infusion

Plasma level of YQ23 will be serially evaluated following dosing of the study drug to determine the Vz

Trial Locations

Locations (2)

Queen Mary Hospital

🇭🇰

Hong Kong, Hong Kong

Prince of Wales Hospital

🇭🇰

Sha Tin, New Territories, Hong Kong

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