Influence of Prior Walking on Postprandial Metabolism and Endothelial Function in South Asian vs White European Women.
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 24
- 试验地点
- 2
- 主要终点
- Triacylglycerol
研究概览
简要总结
The present study will investigate the effect of prior walking on postprandial metabolism and endothelial function in healthy South Asian and White European women.
Participants will complete two, 2-day trials in a random, crossover design separated by at least 3 weeks to control for the menstrual cycle phase.
On day 1, participants will either rest or complete a 60 minute walk at 60% maximal oxygen uptake. On day 2, participants will arrive at 08:00 having fasted overnight and a baseline venous blood sample and endothelial function measurement will be taken. Participants will consume a high-fat breakfast and lunch and 12 subsequent venous blood samples will be taken throughout the day at standardised intervals to measure a variety of coronary heart disease risk markers. A second endothelial function measurement will be completed 2 hours after the breakfast. Blood pressure will be measured every hour.
It is expected that the South Asian participants will have impaired metabolism and endothelial function compared to their European counterparts but the bout of exercise performed on day 1 will mitigate these responses.
详细描述
South Asian individuals have a higher-than-average risk of coronary heart disease. The reasons for this are unclear, but physical inactivity and/or poor responsiveness to exercise may play a role.
Previous research from our laboratory has shown that coronary heart disease risk markers in the postprandial period are elevated in South Asian men, but acute exercise was equally, if not more, effective for reducing these risk markers in South Asian than White European men. However, the effect of acute exercise on coronary heart disease risk markers has not been examined in South Asian women.
Therefore, the purpose of the present study is to compare the effect of acute exercise on endothelial function and coronary heart disease risk markers in women of South Asian versus White European descent.
On visit 1, participants will attend the laboratory to undergo preliminary assessments and to be familiarised with the laboratory environment and study procedures. Specifically, health status, habitual physical activity, dietary habits and anthropometric data (height, weight, waist and hip circumference, body fat) will be collected. Two preliminary exercise tests will be performed as follows: 1) submaximal-incremental treadmill walking test and 2) incremental treadmill running test to exhaustion to measure maximal oxygen uptake.
On visit 2, participants will undergo a magnetic resonance imaging (MRI) scan to quantify regional body composition comprising abdominal subcutaneous adipose tissue, visceral adipose tissue, liver fat percentage, thigh intramuscular adipose tissue and thigh muscle volume.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Prevention
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 40 Years（Adult）
- 性别
- Female
- 接受健康志愿者
- 是
入选标准
- •18 to 40 year old South Asian and White European women;
- •Be able to walk continuously for 1 hour;
- •Weight stable for the past 3 months;
- •Non-smokers;
- •No known contradictions to maximal exertion exercise (e.g., recent musculoskeletal injury, congenital heart disease).
排除标准
- •Musculoskeletal injury that has affected normal ambulation within the last month;
- •Congenital heart disease;
- •Any muscle or bone injuries that do not allow them to walk on a treadmill;
- •Uncontrolled exercise-induced asthma;
- •Coagulation or bleeding disorders;
- •Diabetes (metabolism will be different to non-diabetics potentially skewing the data);
- •Taking any medication that might influence fat metabolism;
- •Taking any medication that might influence blood glucose (e.g., insulin for diabetes);
- •Heart conditions;
- •Dieting or restrained eating behaviours;
- •Weight fluctuation greater than 3 kg in the previous 3 months to study enrolment;
- •A food allergy.
研究组 & 干预措施
Exercise
Participants will walk for 60 minutes at 60% maximal oxygen uptake on day 1. Participants will return the following day and consume a high fat breakfast and lunch.
干预措施: Exercise (Behavioral)
Control
Participants will rest on day 1. Participants will return the following day where they will consume a high fat breakfast and lunch.
结局指标
主要结局
Triacylglycerol
时间窗: Day 1 fasting. Day 2 fasting, 1.5h, 1.75h, 2h, 3h, 4h, 5h, 5.5h, 5.75h, 6h, 7h, 8h and 9h.
Fasting on day 1 and 2. Time-course of plasma triacylglycerol concentrations in response to exercise and/or feeding on day 2.
次要结局
- Endothelial function(Day 1 fasting and 8.75h. Day 2 fasting and 3.5h.)
- Blood pressure(Day 1 fasting. Day 2 fasting and 1h, 2h, 3h, 4h, 5h, 6h, 7h, 8h and 9h.)
- Glucose(Day 1 fasting. Day 2 fasting, 1.5h, 1.75h, 2h, 3h, 4h, 5h, 5.5h, 5.75h, 6h, 7h, 8h and 9h)
- High-density lipoprotein cholesterol(Day 1 fasting. Day 2 fasting.)
- C-Reactive protein(Day 1 fasting. Day 2 fasting, 3h, 6h and 8h.)
- Total cholesterol(Day 1 fasting. Day 2 fasting.)
- Insulin(Day 1 fasting. Day 2 fasting, 1.5h, 2h, 4h, 5h, 5.5h, 7h, 8h.)
- Low-density lipoprotein cholesterol(Day 1 fasting. Day 2 fasting.)
- Non-esterified fatty acids(Day 1 fasting. Day 2 fasting, 1.5h, 2h, 4h, 5h, 5.5h, 7h and 9h.)
- Interleukin-6(Day 1 fasting. Day 2 fasting, 3h, 6h and 8h.)
- Peroxiredoxin-4(Day 1 fasting. Day 2 fasting, 3h, 6h and 8h.)
- Superoxide dismutase 3(Day 1 fasting. Day 2 fasting, 3h, 6h and 8h.)
研究者
Matthew Roberts
Principal Investigator
Loughborough University