Effect of the Amount of Fluoride Toothpaste on Fluoride Availability in the Oral Fluids in Children
- Conditions
- Dental Caries
- Interventions
- Procedure: Toothbrushing
- Registration Number
- NCT03455621
- Lead Sponsor
- University of Campinas, Brazil
- Brief Summary
The aim of the study is to determine the fluoride availability in saliva and dental biofilm after the use of increasing amounts of fluoride toothpaste. Using a crossover and blind design, 15 children (18-42 months old) had their teeth brushed with increasing amounts of fluoride toothpaste. Fluoride concentration in saliva and dental biofilm was determined up to 30 min after brushing.
- Detailed Description
The aim of the study is to determine the fluoride availability in saliva and dental biofilm after the use of increasing amounts of fluoride toothpaste. Children from 18 to 42 months from a community daycare participated. The study had a crossover and blind design. The amounts of toothpastes tested were: 1. A pea-size amount (0.3 g) of a non-F toothpaste; 2. 0.025 g of a F toothpaste (1,100 ppm F, as NaF); 3. 0.05 g of the same F toothpaste; 4. 0.1 g of the same F toothpaste; or 5. 0.3 g (pea-size) of the same F toothpaste. Children were to brush their teeth with the designated amount of toothpaste twice/day from Monday to Friday. In order to allow biofilm accumulation, caregivers were instructed to brush only the occlusal surfaces of primary molars. On Friday morning, at least one hour after breakfast, saliva and one biofilm sample from the right or left hemiarch were collected and analyzed for fluoride concentration. Then, brushing with the respective amount of toothpaste was performed, and saliva was collected 5, 15 and 30 min later. The biofilm from the other side of the mouth was collected after 30 min. Fluoride concentration in saliva and biofilm samples was determined with an ion-specific electrode.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 15
- All children with an informed consent signed by the parents/guardians
- Children with at least on active caries lesion
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- CROSSOVER
- Arm && Interventions
Group Intervention Description 0.025 g of 1,100 ppm F dentifrice Toothbrushing Fluoride dentifrice (1,100 ppm F, as NaF); to be used twice/day. Amount to be used: 0.025 g 0.05 g of 1,100 ppm F dentifrice Toothbrushing Fluoride dentifrice (1,100 ppm F, as NaF); to be used twice/day. Amount to be used: 0.05 g 0.1 g of 1,100 ppm F dentifrice Toothbrushing Fluoride dentifrice (1,100 ppm F, as NaF); to be used twice/day. Amount to be used: 0.1 g Pea-size amount of 1,100 ppm F dentifrice Toothbrushing Fluoride dentifrice (1,100 ppm F, as NaF); to be used twice/day. Amount to be used: 0.3 g (pea-size amount) Pea-size amount of non-F dentifrice Toothbrushing Non-fluoride dentifrice (0 ppm F); to be used twice/day. Amount to be used: 0.3 g (pea-size amount)
- Primary Outcome Measures
Name Time Method Fluoride concentration in dental biofilm (fluid fraction) 30 min after brushing 30 min after brushing Fluoride concentration in saliva before brushing Before brushing (baseline) Fluoride concentration in saliva 15 min after brushing 15 min after brushing Fluoride concentration in saliva 30 min after brushing 30 min after brushing Fluoride concentration in dental biofilm (solid fraction) 30 min after brushing 30 min after brushing Fluoride concentration in saliva 5 min after brushing 5 minutes after brushing Fluoride concentration in dental biofilm (fluid fraction) before brushing Before brushing (baseline) Fluoride concentration in dental biofilm (solid fraction) before brushing Before brushing (baseline)
- Secondary Outcome Measures
Name Time Method