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Predicting Response to Capecitabine in Women With Metastatic Breast Cancer

Not Applicable
Completed
Conditions
Breast Cancer
Interventions
Other: laboratory biomarker analysis
Other: pharmacological study
Registration Number
NCT00953537
Lead Sponsor
Centre Antoine Lacassagne
Brief Summary

RATIONALE: Identifying genes that increase a person's susceptibility to side effects caused by capecitabine may help doctors plan better treatment.

PURPOSE: This clinical trial is studying blood samples in predicting response to capecitabine in women with metastatic breast cancer.

Detailed Description

OBJECTIVES:

Primary

* To determine the sensitivity, specificity, and positive and negative predictive values of dihydrouracil/uracil (UH_2/U) ratio measured before starting treatment on grade 3-4 capecitabine-related toxicity in women with metastatic breast cancer.

Secondary

* To prospectively test the value of the germinal genotype of thymidylate synthase (TS) and methylenetetrahydrofolate reductase (MTHFR) as predictors of resistance to capecitabine.

* To evaluate the practical feasibility of such pre-therapeutic screening.

* To determine the sensitivity, specificity, and positive and negative predictive values of dihydropyrimidine dehydrogenase genotyping on grade 3-4 capecitabine-related toxicity in the first and second courses.

* To evaluate the predictive gain provided by genotyping relative to phenotyping alone.

* To evaluate the influence of TS and MTHFR gene polymorphisms on clinical response and duration of response.

* To evaluate the pharmacokinetics of capecitabine and its metabolites and their relationship with UH_2/U and genotype.

* To evaluate the total cost of pre-therapeutic phenotyping alone and the combination of phenotyping and genotyping.

* To exhaustively analyze the 23 exons of the dihydropyrimidine dehydrogenase (DPYD) gene in patients who developed toxicity.

OUTLINE: This is a multicenter study.

Patients receive oral capecitabine twice daily on days 1-14. Treatment repeats every 21 days in the absence of disease progression or unacceptable toxicity.

Blood samples are collected 8-15 days before the start of treatment and periodically on the first day of treatment for dihydropyrimidine dehydrogenase phenotyping (dihydrouracil/uracil ratio and high performance liquid chromatography analysis), genotyping (4 most relevant single nucleotide polymorphisms), and pharmacokinetic analysis.

Recruitment & Eligibility

Status
COMPLETED
Sex
Female
Target Recruitment
303
Inclusion Criteria

Not provided

Exclusion Criteria

Not provided

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
capecitabinepharmacological study-
capecitabinelaboratory biomarker analysis-
capecitabinecapecitabine-
Primary Outcome Measures
NameTimeMethod
Capecitabine-related toxicity (i.e., hematological, diarrhea, and hand-foot syndrome) recorded during the first and second courses3 months
Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (1)

Centre Antoine Lacassagne

🇫🇷

Nice, France

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