Clinical Research: Effect of Chorioamnionitis on Platelet Activation and Placental Vessel Among Preterm Infants by Wnt-Flt1 Signal Pathway
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 64
- 主要终点
- Placental Microvascular Density(%)
研究概览
简要总结
Objective:The purpose of this study was to explore the effect and mechanism of maternal chorioamnionitis on placental microvasculature and platelet activation among preterm infants by activating Wnt-Flt1 signal pathway .
Methods:With clinical randomized controlled trial (RCT), the cases were matched with 1:1 according to gestational age and divided into 2 groups according to the placental pathology result: chorioamnionitis group and control group. (1) To observe the platelet parameter, birth weight, thrombrocytopenia and hemorrhage complication, such as intracranial hemorrhage, retinal hemorrhage, pulmonary hemorrhage and gastrointestinal hemorrhage. (2) To observe the miscrovascular density (MVD) in placenta, platelet activating factor (CD62p,CD63) and thrombopotetin (TPO) in preterrn infants.The placental MVD was assessed by immunohistochemical method. The platelet activating factors were detected by flow cytometry. TPO was detected by ELISA. (3) To observe Wnt5a, Flt1 and VEGF in placenta and fetal circulation.The measurement data were analyzed by pair t test and conditional logistic regression. Pearson correlation analysis was used for relationship.
详细描述
The exclusive criteria included :(1) born to mother with no any other maternal complications; (2) no congenital abnormalities and neonatal asphyxia; (3) died or discharged within 72 hours.
研究设计
- 研究类型
- Observational
- 观察模型
- Other
- 时间视角
- Prospective
入排标准
- 年龄范围
- 1 Hour 至 —（Child, Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •gestational age<37weeks
- •admitted to the NICU at Guangdong Women and Children Hospital, with a date of birth from June 2016 to December 2016
- •whose mothers were diagnosed chorioamnionitis by placental histopathology
排除标准
- •born to mother with no any other maternal complications
- •no congenital abnormalities and neonatal asphyxia;
- •died or discharged within 72 hours.
结局指标
主要结局
Placental Microvascular Density(%)
时间窗: 24 hours
Placental Microvascular Density (by placental histopathology)
次要结局
- Thrombopoietin (cord blood)(24 hours)
- Flt1 in infants (ng/ml)(24 hours)
- mean platelet volume(MPV)(24 hours)
- Platelet counts (PLT)(24 hours)
- Wnt5 in infants (ng/ml)(24 hours)
- Incidence Hemorrhage Disease (%)(through study completion, an average of half year)
- Platelets Activation Factors in cord blood(24 hours)
- Flt1 in Placenta(IOD)(24 hours)
- VEGF in infants (ng/ml)(24 hours)
- Wnt5a in Placenta(IOD)(24 hours)
- platelet distribution width(PDW)(24 hours)
- VEGF in Placenta(IOD)(24 hours)
研究者
yangjie
Chief of Neontology Dept
Guangdong Women and Children Hospital