The effects of metformin on immune function.
- Conditions
- immunometabolismeffect of metabolism on immunity1002844010018424
- Registration Number
- NL-OMON41133
- Lead Sponsor
- niversitair Medisch Centrum Sint Radboud
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Pending
- Sex
- Not specified
- Target Recruitment
- 12
Inclusion Criteria
healthy, > 18 years of age, no history of TB, no history of cardiac or renal disease
Exclusion Criteria
DM in first degree relatives, obesity (BMI > 25), use of medication
Study & Design
- Study Type
- Observational invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>The primary objective of the study is to evaluate the effects of orally<br /><br>administered metformin on the immune responses of stimulated peripheral blood<br /><br>mononuclear cells (PBMC*s) and macrophages from healthy individuals. The<br /><br>effects of metformin will be determined by measuring the ex-vivo responsiveness<br /><br>of leukocytes to various pathogens and inflammatory stimuli. The primary<br /><br>outcome of this measure is production of cytokines (for example TNF-a, IL-6,<br /><br>IL-10, IL-1β, IL-17, IL-22, IFN-γ secretion by ex vivo stimulated PBMCs.</p><br>
- Secondary Outcome Measures
Name Time Method <p>• Gene expression and transcriptional analysis of samples with a strong focus<br /><br>on immunity, inflammation, epigenetic and metabolic related pathways.<br /><br>• Epigenetic modulation of gene and protein expression by metformin<br /><br>• Metabolic regulation of key energy sensors (AMPK, mTOR, AKT, PKA etc) by<br /><br>metformin</p><br>