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Trial of an Investigational Drug After Rejecting the Relapse of an Allogeneic Transplant

Phase 1
Not yet recruiting
Conditions
Multiple Myeloma
Allogeneic Stem Cell Transplantation
Interventions
Genetic: CARTemis-1
Registration Number
NCT05982275
Lead Sponsor
Fundación Pública Andaluza para la gestión de la Investigación en Sevilla
Brief Summary

Most patients with multiple myeloma (MM) die due to relapse resistant to current treatment, including treatment with anti-B cell maturation antigen (BCMA) CAR-T cells. To overcome some of the potential limitations of this therapy, a new and optimized Anti-BCMA CAR-T has been developed, with the aim of using it in patients with MM who relapse after Allogeneic Haematopoietic Haematopoietic Progenitor. This trial is a prospective phase I/II trial with a 3+3 design. Once Dose Limiting Toxicity is identified, Phase II will begin to assess the efficacy of the procedure.

Detailed Description

This trial is a prospective phase I/II trial with a 3+3 design. Once Dose Limiting Toxicity is identified (up to a maximum dose of 6x106 CAR-T/kg divided over 2 days), phase II of the trial will begin to assess the efficacy of the procedure.

A number of 25 patients will be included to evaluate.

Recruitment & Eligibility

Status
NOT_YET_RECRUITING
Sex
All
Target Recruitment
25
Inclusion Criteria
  1. Patients > 18 years old with a diagnosis of post-allogeneic transplant relapse multiple myeloma.
  2. Measurable disease at the time of screening
  3. Previous treatment with ≥2 lines before and/or after allogeneic transplant.
  4. Patients who are not receiving immunosuppressants at least 1 month before inclusion and who do not have active graft-versus-host disease.
  5. Eastern Cooperative Oncology Group functional status from 0 to 1.
  6. Life expectancy greater than 3 months (at the time of screening)
  7. Patients who give their consent by signing the Informed Consent document.
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Exclusion Criteria
  1. Active systemic immunosuppressive treatment
  2. Patients who have previously received treatment with CAR-T Anti-BCMA.
  3. Absolute lymphocyte count <0.2x109/L
  4. Previous neoplasm, except if it has been in complete remission >3 years, with the exception of skin carcinoma (non-melanoma)
  5. Active infection requiring treatment.
  6. Active HIV, hepatitis B virus or hepatitis C virus infection.
  7. Uncontrolled medical illness.
  8. Severe organic disease that meets any of the following criteria: left ventricular ejection fraction <40%, carbon monoxide diffusion test <40%, glomerular filtration rate <50 ml/min, bilirubin >3 normal value (except Gilbert syndrome).
  9. Previous diagnosis of symptomatic amyloid light chain or primary amyloidosis or POEMS Syndrome.
  10. Pregnant or lactating women.
  11. Women of childbearing age, unable or unwilling to use highly effective contraceptive methods.
  12. Men who cannot or do not wish to use highly effective contraceptive methods. The partner of the male participants, if they are women of childbearing age, must also use highly effective contraceptive methods during the study period.
  13. Contraindication to receive lymphodepleting chemotherapy.
  14. Patients with known hypersensitivity to the active ingredients or any of the excipients of the product to be infused.
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Study & Design

Study Type
INTERVENTIONAL
Study Design
SEQUENTIAL
Arm && Interventions
GroupInterventionDescription
CARTemis-1CARTemis-1Dose escalation sequential cohorts CARTemis-1 will be self-administered intravenously one or two days, depending on the dose administered.
Primary Outcome Measures
NameTimeMethod
Maximum tolerated doseUp to 30 days

To determine the maximum tolerated dose of CarTemis-1

Purity of CARTemis-1Immediately after infusion

Number of cases in which, after performing apheresis, the manufacturing process is completed and CARTemis-1 cells are infused

Suspected Unexpected Serious Adverse ReactionUp to 36 months after treatment administration

Describe the adverse event that occurs in a clinical trial subject, which is assessed by the sponsor and or study investigator as being unexpected, serious and as having a reasonable possibility of a causal relationship with the study drug.

Infusion reactionsImmediately after intravenous administration of CARTemis-1

To appearance of any of the following symptoms after intravenous administration of CARTemis-1: cardiac events, chills, dyspnea, fatigue, sudden hypertension, hypotension, nausea, pain, fever, skin rash, and urticaria.

Tumor lysis syndromeUp to 30 days after treatment administration

To increase nucleic acids, potassium, and phosphate in the blood

Serious Adverse EventUp to 36 months after treatment administration

Type, incidence, severity (graded by the National Cancer Institute Common Terminology Criteria for Adverse Events, Version 5.0), timing, intensity, and relatedness of adverse events).

Secondary Outcome Measures
NameTimeMethod
Persistence of CARTemis-1Peripheral blood:days 3,7,10,14,17 (only in cohort 3 and 4),21,30,56,90,128 and 156, and months 6,9,12,15,18,24 and relapse or month 36 post-infusion; Marrow: 1,3,6,12,18 and 24 months post-infusion and in the progression or month

Presence of CARTemis-1 in peripheral blood and bone marrow

Expression of B cell maturation antigen (BCMA)Screening and at the time of follow up when a relapse occurs, an average after 1 year

Genetic expression of BCMA at selection and at relapse in patients

Overall response rate3, 6 and 12 months after CARTemis-1 infusion

Overall response rate as assessed by criteria of the International Myeloma Working Group

Progression-free survival.Up to 36 months after treatment administration

Quantification of time between administration of CARTemis-1 and disease progression or death

Number of Participants with cytopeniasDuring the first 90 days after administration of CARTemis-1

Neutropenias or thrombopenias

Overall survivalUp to 36 months after treatment administration

the time elapsed between the infusion of CARTemis-1 and the patient's death from any cause.

CART cell qualityDuring the manufacturing process, at the time of infusion and at Month+1, Month+3, Month+6, Month+12, Month+18 and Month+24 post-infusion

Evaluation of the biological characteristics of CARTemis-1 performed by flow cytometry to identify the optimal time of expansion as well as to study the dynamics of CAR T cells during the manufacturing process and how the manufacturing impacts on CAR T cell features and, therefore, CAR T cell quality.

Time to best responseUp to 36 months after treatment administration

Duration of clinical response as assessed by Urinalysis (immunofixation, proteinuria and 24-h urine proteinogram), Blood tests (total immunoglobulin A, G and M, immunofixation and proteinogram (M component) in serum; serum free light chains), Bone Marrow Aspirate and Positron Emission Tomography

Negative Minimum Residual Disease Rate3, 6 and 12 months after CARTemis-1 infusion

Residual amount of malignant cells in the bone marrow

Response rate of extramedullary disease3 months after CARTemis-1 infusion

To measure of the metabolic activity of the human body by Positron Emission Tomography

B cell maturation antigen (BCMA) levelsScreening, Day -5, Day 0, +1, +3, +7 y +28 and months +3, +6, +12, +18 and +24

Serum soluble BCMA levels pre-treatment and during treatment

Number of Participants with prolonged cytopeniasUp to 12 months after treatment administration

Neutropenias or thrombopenias (grade ≥3) for more than 6 weeks

Duration of clinical responseScreening, day -5, -4, -3, +28, +56, +100 and months +4, +5, 6, +7, +8, +9, +10, +11, +12, +15 , +18, +21, +27, +30, +33, +36 or progression

Duration of clinical response as assessed by Urinalysis (immunofixation, proteinuria and 24-h urine proteinogram), Blood tests (total immunoglobulin A, G and M, immunofixation and proteinogram (M component) in serum; serum free light chains), Bone Marrow Aspirate and Positron Emission Tomography.

Time to complete remissionUp to 36 months after treatment administration

Duration of clinical response as assessed by Urinalysis (immunofixation, proteinuria and 24-h urine proteinogram), Blood tests (total immunoglobulin A, G and M, immunofixation and proteinogram (M component) in serum; serum free light chains), Bone Marrow Aspirate and Positron Emission Tomography

Trial Locations

Locations (5)

Hospital Universitario Marques de Valdecilla

🇪🇸

Santander, Cantabria, Spain

Hospital Santa Creu i Sant Pau

🇪🇸

Barcelona, Spain

José Antonio Pérez Simón

🇪🇸

Sevilla, Spain

Complejo asistencial universitario de Salamanca

🇪🇸

Salamanca, Spain

Hospital Clínico de Valencia

🇪🇸

Valencia, Spain

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