Regulation of Muscle ATP Synthase Beta Subunit Metabolism in Obesity
试验速览
- 阶段
- 1 期
- 状态
- 已完成
- 发起方
- Mayo Clinic
- 入组人数
- 84
- 试验地点
- 1
- 主要终点
- Rate of synthesis of muscle proteins (mixed muscle proteins, mitochondrial proteins, β-F1-ATPase)
研究概览
简要总结
Obesity is associated with reduced adenosine triphosphate (ATP) turnover in skeletal muscle, a condition that can impair muscle metabolism. The proposed research will discover mechanisms responsible for decreased content in mitochondrial proteins as well as in protein β-F1-ATPase, which is directly responsible for ATP assembly, in the muscle of obese individuals. This research will further examine the effectiveness of interventions, such as increased plasma amino acid availability and exercise, to increase the rate of production of mitochondrial proteins as well as that of β-F1-ATPase in the muscle of obese individuals. The findings will help to develop appropriate interventions to improve muscle ATP turnover and metabolism in obese people.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- Single (Participant)
入排标准
- 年龄范围
- 18 Years 至 50 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Body mass index (BMI): lean, 19-26 kg/m2; obese, 30-40 kg/m2
- •Availability of transportation
- •Ability to sign informed consent form
排除标准
- •Medication or supplements (i.e. amino acids, protein) known to affect protein metabolism
- •Presence of acute illness
- •History of liver disease
- •Uncontrolled metabolic disease
- •ECG documented abnormalities, atrial fibrillation, history of syncope, limiting or unstable angina, or congestive heart failure
- •Chronically elevated blood pressure (systolic, >140 mmHg; diastolic, >100 mmHg)
- •Cardiac pacemaker or other medical device implanted in the body
- •Low hemoglobin or hematocrit
- •Current participation in a weight-loss regimen, including extreme dietary practices
- •Use of anabolic steroids or corticosteroids (within 3 months)
研究组 & 干预措施
Amino Acid Infusion in Lean
Amino Acid Infusion in Lean
干预措施: Increase in the levels of plasma amino acids (Other)
Amino acid Infusion in Obese
Amino acid Infusion in Obese
干预措施: Increase in the levels of plasma amino acids (Other)
Exercise in lean
Exercise in lean
干预措施: Aerobic exercise (Other)
Exercise in Obese
Exercise in Obese
干预措施: Aerobic exercise (Other)
结局指标
主要结局
Rate of synthesis of muscle proteins (mixed muscle proteins, mitochondrial proteins, β-F1-ATPase)
时间窗: Measured during a 9-hour infusion study
次要结局
- β-F1-ATPase mRNA expression; PGC-1 expression(Measured during a 9-hour infusion study)