Bioavailability and Bioactivity of Red Wine Melatonin in Humans
- Conditions
- Bioavailability
- Interventions
- Other: Red wine with melatoninOther: Red wine without melatonin
- Registration Number
- NCT02767102
- Lead Sponsor
- University of Milan
- Brief Summary
Melatonin (MLT) is a bioactive compound in red wine. The investigators will investigate the serum and salivary concentrations and antioxidant power of MLT after the intake of a glass of red wine. Twelve healthy volunteers were recruited to receive both 125 mL of red wine rich in MLT (10 ng mL-1) and without MLT (placebo), via a randomized, cross-over, and double-blind design. Serum and salivary MLT will be measured by liquid chromatography coupled to mass spectrometry (LC-MS) and enzyme-linked immunosorbent (ELISA), respectively. The ferric ion reducing antioxidant power (FRAP) will be analyzed.
- Detailed Description
Melatonin (MLT) is a bioactive compound in red wine. No information is currently available on serum and salivary concentrations of MLT after drinking a glass of red wine, containing this indoleamine. Hence, in this study, the investigators will investigate the levels of MLT, in serum and saliva, soon after the intake of the MLT-rich red wine, and, correspondingly, they will verify the antioxidant power of the same biological fluids.
This will be a randomized, crossover, double-blind, placebo-controlled study with two treatment arms. Twelve healthy volunteers will be recruited to receive both 125 mL of red wine rich in MLT (10 ng mL-1) and without MLT (placebo), via a randomized, cross-over, and double-blind design.
Serum and salivary MLT will be measured by liquid chromatography coupled to mass spectrometry (LC-MS) and enzyme-linked immunosorbent (ELISA), respectively. The ferric ion reducing antioxidant power (FRAP) will be also analyzed.
For serum analyses, samples will be collected at 0, 30, 60 and 90 min. For saliva analyses, samples will be collected at 0, 45 and 120 min.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 12
- to be healthy
- to have normal weight (BMI 18.5-25.0)
- to voluntarily accept to participate to the study.
- pregnancy and lactation for women;
- supplementation with MLT and/or any other dietary supplement (vitamins, antioxidants, botanicals, phytochemicals)
- systemic and chronic-degenerative diseases
- abnormal hematological parameters
- heavy smoking and alcohol drinking
- high-intensity physical activity
- under pharmacological treatment
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- CROSSOVER
- Arm && Interventions
Group Intervention Description Red wine with Melatonin Red wine with melatonin Rosso di Marco is a wine without MLT (previous screening). This wine will be enriched with 10 ng mL-1 MLT (MLT+) and used as experimental wine. Red wine without Melatonin Red wine without melatonin Rosso di Marco is a wine without MLT (previous screening). This wine will be used as placebo treatment (PLC).
- Primary Outcome Measures
Name Time Method Serum and salivary melatonin after red wine intake (with or without MLT) Time-course of the serum melatonin levels in healthy volunteer from 0 min to 90 min after administration of red wine. Time-course of the salivary melatonin levels in healthy volunteer from 0 min to 120 min after administration of red wine. LC-MS analyses for serum and ELISA analyses for saliva
- Secondary Outcome Measures
Name Time Method Antioxidant power of saliva and serum after red wine intake (with or without MLT) Time-course of the serum antioxidant power in healthy volunteer from 0 min to 90 min after administration of red wine. Time-course of the salivary antioxidant power in healthy volunteer from 0 min to 120 min after administration of red wine. FRAP analyses
Trial Locations
- Locations (1)
University of Milan
🇮🇹Milano, Italy