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Use of Erythropoietin to Expand Regulatory T Cells in Autoimmune Liver Disease

Early Phase 1
Completed
Conditions
Autoimmune Hepatitis
Interventions
Registration Number
NCT03842254
Lead Sponsor
Northwestern University
Brief Summary

This study evaluates the effect of erythropoietin on the number and function of regulatory T cells in adults with autoimmune hepatitis. Participants will receive a single dose of erythropoietin, and then the investigators will collect blood at different time points for analysis of regulatory T cell number and function.

Detailed Description

There is data from the laboratory that erythropoietin helps stimulate regulatory T cells, a type of immune cell which is thought to combat autoimmunity, but this study will look at whether it does the same thing in adults with autoimmune hepatitis.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
6
Inclusion Criteria
  • Diagnosis of autoimmune liver diseases (autoimmune hepatitis, autoimmune cholangiopathy, or primary biliary cirrhosis or primary sclerosing cholangitis with hepatic autoimmune liver disease or overlap syndrome) confirmed on liver biopsy
  • Use of immunosuppressive therapy (prednisone, azathioprine, 6-mercaptopurine, mycophenolate mofetil) for the treatment of the autoimmune liver disease
  • Stable immunosuppression regimen at least 6 months prior to enrollment
  • Ability to provide verbal and written informed consent
Exclusion Criteria
  • Diagnosis of decompensated cirrhosis (defined by presence of ascites, varices, encephalopathy)
  • Hemoglobin above average normal value (15.7 g/dL in men, 13.8 g/dL in women)
  • Alanine aminotransferase greater than 2 times upper limit of normal (33 IU/L in men and 25 IU/L in women)
  • Uncontrolled hypertension with systolic blood pressure greater than or equal to 160 or diastolic blood pressure greater than or equal to 100
  • End-stage renal disease on hemodialysis
  • History of venous thromboembolism including deep vein thromboses or pulmonary emboli
  • History of stroke
  • History of heart failure
  • History of seizure disorder
  • History of significant cardiovascular disease including a history of myocardial infarction
  • Active malignancy (untreated or undergoing therapy)
  • History of pure red cell aplasia
  • History of intolerance or allergy to erythropoietin
  • Known hypersensitivity to mammalian cell-derived products
  • Known hypersensitivity to human albumin
  • Presence of vascular access
  • Prior recipient of erythropoietin within 12 weeks of the study
  • Patient unable to provide consent including infants, children, teenagers, prisoners, cognitively impaired adults
  • Non-English speaking
  • Pregnancy

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
Single armErythropoietinSingle dose of erythropoietin 10,000 units to be administered subcutaneously at time of enrollment.
Primary Outcome Measures
NameTimeMethod
Change in number of regulatory T cellsAt time of enrollment, then at 2 weeks, 4 weeks, and 12 weeks.

Calculated relative to baseline collected at time of enrollment

Secondary Outcome Measures
NameTimeMethod
Change in number of effector T cells after a single dose of erythropoietinAt time of enrollment, then at 2 weeks, 4 weeks, and 12 weeks.

Calculated relative to baseline collected at time of enrollment

Change in cytokine production by the T cells in response to ex vivo stimulationAt time of enrollment, then at 2 weeks, 4 weeks, and 12 weeks.

Calculated relative to baseline collected at time of enrollment

Trial Locations

Locations (2)

Northwestern Medicine

🇺🇸

Chicago, Illinois, United States

Mount Sinai

🇺🇸

New York, New York, United States

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