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Phase II Trial of Disulfiram With Copper in Metastatic Breast Cancer

Phase 2
Recruiting
Conditions
Metastatic Breast Cancer
Breast Neoplasm Female
Interventions
Registration Number
NCT03323346
Lead Sponsor
The Institute of Molecular and Translational Medicine, Czech Republic
Brief Summary

The aim of the study is to establish clinical evidence for introducing disulfiram and cooper as an active therapy for metastatic breast cancer upon failure of conventional systemic and/or locoregional therapies.

Analyses of the following objectives will be performed in the population of patients with metastatic breast cancer:

Primary efficacy objective:

To evaluate the efficacy of the treatment by assessment of:

* clinical response rate (RR)

* clinical benefit rate (CBR)

Secondary efficacy objectives:

To evaluate the efficacy of the treatment by assessment of:

* time to progression (TTP)

* overall survival (OS)

Pharmacokinetic objectives:

• to determine pharmacokinetic parameters for disulfiram and its active metabolites administered in combination with copper supplements in cancer patient population

Safety objectives:

• to describe safety profile of disulfiram administered in combination with copper supplements

Exploratory objectives:

Parallel analysis to assess (identify) potential candidate surrogate biomarkers of disulfiram efficacy, as well as identification (using proteomic, biochemical and molecular genetic studies) of potential predictive biomarkers of disulfiram sensitivity or resistance will be performed. Surrogate biomarker analysis will focus on in vivo ubiquitin-proteosomal system inhibition, cell cycle and DNA damage.

Detailed Description

Inclusion criteria:

1. Patients with stage IV breast cancer with metastases demonstrated by appropriate imaging techniques

2. Histologically or cytologically confirmed tumor

3. Age of 18 years or more

4. Eastern Cooperative Oncology Group (ECOG) performance status of 0 - 2

5. Patients have failed, untolerated or refused standard therapeutic modalities

6. Not received systemic anticancer therapy or radiation or had major surgery in last 2 weeks

7. Not currently participating in another study

8. Anticipated survival of at least 2 months

9. Baseline aspartate aminotransferase (AST) and alanine aminotransferase (ALT) not greater than 2.5 X upper institutional limit

10. Serum copper within normal limits

11. Serum ceruloplasmin \> 17 mg/dL

12. Able and willing to sign informed consent and to comply with study procedures

13. Able to ingest oral medications

14. No known allergy to disulfiram or copper

15. Willing to refrain from ingestion of alcoholic beverages while on the study

Exclusion criteria:

1. Participation in another clinical trial of a therapeutic drug during the past 14 days

2. Addiction to alcohol or drugs

3. Baseline AST or ALT greater than 2.5 X upper institutional limit

4. Unable to ingest oral medications

5. Unable to undergo CT/SPECT scanning because of inability to lie recumbent in the scanner

6. Actively receiving cytotoxic cancer chemotherapy agents

7. Anticipated survival of less than 2 months

8. Women of child-bearing potential who are not using a commonly accepted effective means of contraception; women of child-bearing potential will have negative pregnancy test before enrollment

9. History of active liver disease, including chronic active hepatitis, viral hepatitis (hepatitis B, C and CMV), cholestatic jaundice of any etiology, toxic hepatitis, or cholestatic hepatitis or jaundice with bilirubin greater than 2.0 X upper institutional limit

10. History of Wilson's disease or family member with Wilson's disease

11. History of hemochromatosis or family member with hemochromatosis

12. History of other iron overload syndrome such as hemochromatosis

13. Need for metronidazole, warfarin and/or theophylline medication, the metabolism of which is likely influenced by disulfiram

14. Pregnant women and nursing mothers are not allowed to enroll on this study

15. Patients who are taking medications metabolized by cytochrome P450 2E1, including chlorzoxazone or halothane and its derivatives

Recruitment & Eligibility

Status
RECRUITING
Sex
Female
Target Recruitment
150
Inclusion Criteria
  1. Patients with stage IV breast cancer with metastases demonstrated by appropriate imaging techniques (computer tomography - CT, positron emission tomography - PET or PET/CT, MRI, ultrasound, etc.)
  2. Histologically or cytologically confirmed tumor
  3. Age of 18 years or more
  4. ECOG performance status of 0 - 2
  5. Patients have failed, untolerated or refused standard therapeutic modalities
  6. Not received systemic anticancer therapy or radiation or had major surgery in last 2 weeks
  7. Not currently participating in another study
  8. Anticipated survival of at least 2 months
  9. Baseline AST and ALT not greater than 2.5 X upper institutional limit
  10. Serum copper within normal limits
  11. Serum ceruloplasmin > 17 mg/dL
  12. Able and willing to sign informed consent and to comply with study procedures
  13. Able to ingest oral medications
  14. No known allergy to disulfiram or copper
  15. Willing to refrain from ingestion of alcoholic beverages while on the study
Exclusion Criteria
  1. Participation in another clinical trial of a therapeutic drug during the past 14 days
  2. Addiction to alcohol or drugs
  3. Baseline AST or ALT greater than 2.5 X upper institutional limit
  4. Unable to ingest oral medications
  5. Unable to undergo CT/SPECT scanning because of inability to lie recumbent in the scanner
  6. Actively receiving cytotoxic cancer chemotherapy agents
  7. Anticipated survival of less than 2 months
  8. Women of child-bearing potential who are not using a commonly accepted effective means of contraception; women of child-bearing potential will have negative pregnancy test before enrollment
  9. History of active liver disease, including chronic active hepatitis, viral hepatitis (hepatitis B, C and CMV), cholestatic jaundice of any etiology, toxic hepatitis, or cholestatic hepatitis or jaundice with bilirubin greater than 2.0 X upper institutional limit
  10. History of Wilson's disease or family member with Wilson's disease
  11. History of hemochromatosis or family member with hemochromatosis
  12. History of other iron overload syndrome such as hemochromatosis
  13. Need for metronidazole, warfarin and/or theophylline medication, the metabolism of which is likely influenced by disulfiram
  14. Pregnant women and nursing mothers are not allowed to enroll on this study
  15. Patients who are taking medications metabolized by cytochrome P450 2E1, including chlorzoxazone or halothane and its derivatives

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
Disulfiram with copperDisulfiramPatients will take one pill of disulfiram (Antabus) daily at a dose of 400 mg continually during the treatment phase (from day 0 till End of treatment Visit). In case of intolerance, lower dose up to 200 mg per day is allowed. Patients will take disulfiram after their evening meal. Patients will avoid alcohol and other disulfiram-drug interactions will be considered. Copper supplementation will be given separately from disulfiram; in the morning with patients´breakfast. Patients will take one pill of copper dietary supplement (for instance Copper Star, STARLIFE) corresponding to 2 mg of elementary copper.
Primary Outcome Measures
NameTimeMethod
Clinical response rate (RR)From date of randomization until the date of first documented progression or date of death from any cause, whichever came first, assessed up to 60 months

sum of complete and partial responses (CR+PR)

Clinical benefit rate (CBR)From date of randomization until the date of first documented progression or date of death from any cause, whichever came first, assessed up to 60 months

sum of complete, partial responses and stable diseases (CR+PR)CR+PR+SD)

Secondary Outcome Measures
NameTimeMethod
Overall survival (OS)From date of randomization until the date of first documented progression or date of death from any cause, whichever came first, assessed up to 60 months

overall survival (OS) in months

The pharmacokinetic (PK) characteristicsDay 0 = at first administration of the drug

Cmax

The pharmacokinetic (PK) characteristic - λzDay 0 = at first administration of the drug

λz (Lambda-z) - Individual estimate of the terminal elimination rate constant, calculated using log-linear regression of the terminal portions of the plasma concentration-versus-time curves

Time to progression (TTP)From date of randomization until the date of first documented progression or date of death from any cause, whichever came first, assessed up to 60 months

time to progression (TTP) in months

The pharmacokinetic (PK) characteristic - T-maxDay 0 = at first administration of the drug

T-max - Time to reach maximum concentration

The pharmacokinetic (PK) characteristic - T1/2Day 0 = at first administration of the drug

T1/2 - Apparent terminal elimination half-life time

Number of participants with treatment-related adverse events as assessed by CTCAE v4.0From date of randomization until the date of first documented progression or date of death from any cause, whichever came first, assessed up to 60 months

Number of participants with treatment-related adverse events analyzed as cumulative burden at every 6 months until study termination.

The pharmacokinetic (PK) characteristic - Area Under Curve (AUC)Day 0 = at first administration of the drug

AUC - The area under the plasma concentration over the time

Trial Locations

Locations (1)

University Hospital Olomouc

🇨🇿

Olomouc, Czechia

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