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Large-scale ex Vivo Expansion and Characterization of Two Kinds of MSC From Knee for Clinical Application

Completed
Conditions
Knee Injuries
Registration Number
NCT02386163
Lead Sponsor
EMO Biomedicine Corporation
Brief Summary

According to many researches, mesenchymal stem cells (MSC) isolated from synovial membrane and infrapatellar fat pad have higher ex vivo proliferation capacity, multipotency and ability to undergo chondrogenesis.

Based on this concept, the investigators plan to get two removed tissues (synovial membrane and infrapatellar fat pad) from patients who have an operation of TKR (Total Knee Replacement). The investigators are going to isolate and expand MSC from these two tissues respectively, and then compare their characterization and potential for clinical application. In the other hand, the investigators plan to build techniques and procedures which comply with "Good Tissue Practices (GTP)".

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
6
Inclusion Criteria

Patient who have an total knee replacement surgery

Exclusion Criteria

Patient who don't sign the agreement

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
Compare MSCs from synovial membrane and infrapatellar fat pad2 months

Comparison items include doubling time, phenotype, chondrogenesis and biological activity.

Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (1)

Far Eastern Memorial Hospital

🇨🇳

New Taipei City, Taiwan

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