Acute Impact of Different Foods and Beverages on Exercise Induced Oxidative Stress and Inflammation

Not Applicable

Completed

• Conditions: Oxidative Stress Induction; Oxidative Stress; Inflammatory Response

• Registration Number: NCT05242978
• Lead Sponsor: University of Vienna

Brief Summary

The aim of this research project is to explore the acute impact of specific foods and beverages, which have been shown to be associated with inflammatory processes, mainly in epidemiological studies, on inflammatory and oxidative stress parameters in healthy humans following a high intensity physical workout.

Detailed Description

The aim of this research project is to explore the acute impact of specific foods and beverages, which have been shown to be associated with inflammatory processes, mainly in epidemiological studies, on inflammatory and oxidative stress parameters in healthy humans following a high intensity physical workout. The choice of food will mainly be based on the food groups from the Empirical Dietary Inflammatory Index. For that purpose, forty-two healthy volunteers will be recruited to perform several interventions in order to assess the impact of different nutrient groups or single- foods on oxidative and inflammatory markers. They will be divided into two groups (beverage consumption, n=20 \& plantbased foods, n=22). The intense physical activity serves to induce a physiological stress response in the body, known to be accompanied by an increase in oxidative and inflammatory stress parameters. The idea is that anti- inflammatory or antioxidant nutrients could potentially attenuate this stress response in dependence on their anti- inflammatory or antioxidant potential. The operability of this concept has already been tested. The main benefit of this approach is to get a deeper insight into short term effects of single foods or beverages on inflammatory and prooxidative processes in humans by using short term intense physical activity as a stress model. One major practical aspect of this approach is to assess dietary components with respect to their pro- or anti-inflammatory-/oxidative stress potential regarding e.g. non-communicable diseases such as type 2 diabetes, coronary heart disease or the metabolic syndrome. Furthermore, it could help to reduce inflammation or oxidative stress during or following high intense physical exercise in order to reduce the stress response during training or competition in order to enhance regeneration and to foster the training gain in sports.

Study Timeline

• Study First Submitted: 2022-01-26
• Study First Submitted QC: 2022-02-07
• Study First Posted: 2022-02-16
• Study Start: 2022-05-17
• Primary Completion: 2024-04-30
• Study Completion: 2024-04-30
• Status Verified: 2024-11
• Last Update Submitted: 2025-07-01
• Last Update Posted: 2025-07-04

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Female
• Target Recruitment: 42

Inclusion Criteria

• female human subjects
• aged 18-40 years
• no chronical diseases
• not more than 120 minutes of physical activity per week
• no regular resistance training within the past 6 months
• BMI 18.5 - 24.99 kg/m²
• body fat percentage of at least 20%

Exclusion Criteria

• contraindications against physical activity
• anemia
• fructose or lactose intolerance
• smoking
• medications with influence on ROS levels or inflammation
• allergy or intolerance against intervention food/beverages.

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Primary Outcome Measures

Name	Time	Method
ROS change	change from baseline to 2.5 hours	ROS (change from baseline = after overnight fasting to post exercise (=after 2.5 hours)
IL-6 change	change from baseline to 2.5 hours	IL-6 (change from baseline = after overnight fasting to post exercise (=after 2.5 hours)

Secondary Outcome Measures

Name	Time	Method
estradiol	baseline	17-beta-estradiol
DNA damage (comet assay	change from baseline to 2.5 hours	comet assay - wholeblood
MDA change	change from baseline to 2.5 hours	MDA (change from baseline = after overnight fasting to post exercise (=after 2.5 hours)
hs-CRP change	change from baseline to 2.5 hours	hs-CRP (change from baseline = after overnight fasting to post exercise (=after 2.5 hours)
FRAP	change from baseline to 2.5 hours	Ferric Reducing Antioxidant Power Assay
Polyphenol Content	change from baseline to 2.5 hours	Polyphenol Content Plasma
Lipidomics	change from baseline to 2.5 hours	oxLDL
Protein Carbonyls	change from baseline to 2.5 hours	Protein Carbonyl Assay

Trial Locations

Locations (1)

University of Vienna; 🇦🇹 Vienna, Austria