The Effect of Exercise on Myokine Production in Aging Persons
- Conditions
- ExerciseAging
- Interventions
- Other: Exercise trainingBehavioral: QuestionnaireProcedure: Blood sampleProcedure: Percutaneous muscle biopsy
- Registration Number
- NCT05571709
- Lead Sponsor
- Hasselt University
- Brief Summary
The incidence of age-related diseases, such as cancer and cardiovascular diseases, rapidly rises. These days, age-related diseases cause the majority of all healthcare expenses. During ageing senescent cells secrete a range of harmful inflammatory signals, which leads to chronic inflammation (inflamm-aging) and accelerates aging throughout the rest of the body. Interestingly, people who regularly exercise show less signs of inflamm-aging compared to people with a sedentary lifestyle. Molecules secreted by skeletal muscles, called myokines, may play an important role. However, the underlying molecular mechanisms remain unknown. This pilot study aims to investigate if an acute bout of resistance training is an appropriate tool to induce an increase in the release of muscle derived myokines in aged persons.
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- RECRUITING
- Sex
- All
- Target Recruitment
- 20
- Healthy persons
-
Smoking
-
Infection < 2 weeks ago
-
Received a vaccine < 2 weeks ago
-
Allergy to Lidocain
-
Immune deficiency
-
Presence of a tumour or in treatment
-
Usage of anti-coagulant medication
-
Presence of following conditions:
- Haemophilia
- Auto-immune disease
- Cardiovascular disease
- Musculoskeletal diseases which refrains the patient from exercising
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Arm && Interventions
Group Intervention Description Aged individuals who stay sedentary Blood sample Aged individuals will remain seated for 30 minutes. Aged individuals who perform exercise training Exercise training Aged individuals will perform an acute bout of resistance trainig (30 minutes). This training is focused on the lower limbs. Aged individuals who stay sedentary Questionnaire Aged individuals will remain seated for 30 minutes. Aged individuals who perform exercise training Blood sample Aged individuals will perform an acute bout of resistance trainig (30 minutes). This training is focused on the lower limbs. Aged individuals who perform exercise training Questionnaire Aged individuals will perform an acute bout of resistance trainig (30 minutes). This training is focused on the lower limbs. Aged individuals who perform exercise training Percutaneous muscle biopsy Aged individuals will perform an acute bout of resistance trainig (30 minutes). This training is focused on the lower limbs. Aged individuals who stay sedentary Percutaneous muscle biopsy Aged individuals will remain seated for 30 minutes.
- Primary Outcome Measures
Name Time Method Cytokine concentration Day 2 Measurement of multiple cytokines in blood (for example: IL-6, IL-15, IL-4, irisin...) with ELISA's or multiplex assays
Myokine production Day 2 Immunohistochemistry staining (fluorescent) of multiple myokines (for example: IL-6, IL-15, IL-4, irisin..) and aging markers (p16,p21...) on muscle tissue
Myokine protein levels Day 2 Western blotting of multiple myokines in muscle tissue
Myokine gene expression Day 2 Quantitative PCR (polymerase chain reaction) of markers in muscle tissue
Immune parameters Day 2 Flow cytometry panels of immune parameters in muscle tissue
- Secondary Outcome Measures
Name Time Method Resting Heart Rate Day 1 Measured using an electronic sphygmomanometer
Blood pressure Day 1 The force of circulating blood on the walls of the arteries. Measured using an electronic sphygmomanometer
International Physical Activity Questionnaire (Short form) Day 1 Measures the subjective level of activity of the last seven days. 7 questions regarding amount of time spent on certain activities.
Trial Locations
- Locations (1)
Hasselt University
🇧🇪Diepenbeek, Limburg, Belgium