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480 STUDY: Phase 2b Study of Locteron Plus Ribavirin to Treat Hepatitis C Virus (HCV)

Phase 2
Completed
Conditions
Hepatitis C, Chronic
Interventions
Registration Number
NCT00953589
Lead Sponsor
Biolex Therapeutics, Inc.
Brief Summary

The purpose of this 12-week study was to assess in subjects with chronic hepatitis C (treatment-naïve, genotype 1) receiving weight-based doses of ribavirin the early virologic response to the 480 ug dose level of Locteron™, dosed every 2 weeks, in comparison with 1.5 ug/kg PEG-Intron™ dosed weekly.

Detailed Description

The aim of the 480 STUDY was to compare efficacy and safety of 480ug Locteron dosed every other week to 1.5 ug/kg PegIntron dosed weekly in treatment-naïve genotype-1 chronic HCV subjects treated with weight-based ribavirin. This 12-week study was comprised of two panels (Panel A and Panel B). The designs of both panels were identical. HCV RNA was measured weekly for three weeks and then every other week. Adverse events including flu-like events and depression were collected during weekly clinic visits for 12 weeks. Flu-like events were also collected daily for 12 weeks by subject self-report using the internet (ePRO). Beck Depression Inventory (BDI) and Short Form-36 scores were measured at baseline and monthly through Week 12.

In Panel A of 480 STUDY, 42 treatment-naïve subjects with chronic genotype-1 HCV in Bulgaria and Romania were randomized and dosed with either Locteron q2weeks or weekly PegIntron, both in combination with weight-based ribavirin (13). In Panel A, 19 subjects received 480ug Locteron and 23 subjects received PegIntron.

In Panel B of 480 STUDY, 32 treatment-naïve subjects with genotype-1 HCV in Israel were randomized and dosed with either Locteron q2weeks or weekly PegIntron, both in combination with weight-based ribavirin (13). In Panel B, 16 subjects received 480ug Locteron and 16 subjects received PegIntron.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
74
Inclusion Criteria
  • Male and female subjects 18 through 69 years of age, inclusive
  • Chronic hepatitis C genotype 1
  • HCV ribonucleic acid (RNA) level > 10,000 IU/mL (by RT-PCR) at screening
  • Creatine clearance ≥ 50 mL/min
  • Neutrophil count > 1500 cells/mm3
  • Platelet count > 90,000/mm3
  • Hemoglobin > 12 g/dL for females and > 13 g/dL for males
  • Female subjects of child-bearing potential agreeing to use dual methods for contraception
  • Male subjects with female sexual partners agreeing to use effective birth control methods
  • Negative serum pregnancy test for women of child-bearing potential
  • Compensated liver disease defined as INR < 1.5, conjugated bilirubin < 1.5 x ULN, serum albumin > 3.0 g/dL
  • Histologic evidence of Chronic Hepatitis C (CHC) (inflammation, fibrosis and/or cirrhosis on a standardized histologic grading system) as shown by biopsy within 2 years of screening or agrees to have a liver biopsy performed prior to randomization.
Exclusion Criteria
  • Prior antiviral treatment for hepatitis C
  • Co-infection with HIV or hepatitis B virus
  • Subjects with a body mass index (BMI) above 32 kg/m2
  • Current or prior history of clinical hepatic decompensation
  • Evidence of HCC
  • Uncontrolled diabetes mellitus as evidenced by HbA1C ≥ 8.5% at screening
  • Known hypersensitivity to interferon alfa or ribavirin
  • Chronic liver disease other than HCV
  • Clinically significant hemoglobinopathy
  • History of moderate, severe or uncontrolled psychiatric disease including depression and prior suicide attempts
  • History of immune-mediated disease
  • Significant renal or neurological disease
  • Severe degree (> GOLD stage III) of chronic pulmonary disease (COPD) or active, severe asthma
  • Subjects with severe cardiac disease
  • History of significant central nervous system (including CNS trauma) or seizure disorders
  • Cancer within the last 5 years, or previous cancer with a high risk of recurrence
  • History of solid organ or bone marrow transplantation
  • Clinical or laboratory evidence of uncontrolled thyroid disease, e.g., by thyroid stimulating hormone (TSH) level > 1.2 x upper limit of normal
  • Clinically significant retinopathy; this needs to have been excluded by an eye exam performed by an ophthalmologist within the last 6 months prior to screening for subjects with hypertension or diabetes mellitus
  • Drug abuse or alcohol consumption within the last 6 months which, in the opinion of the investigator, may affect study participation or outcome. Subjects in a supervised methadone treatment program on a stable regimen for > 6 months may be considered
  • Taken any experimental agent within 12 weeks prior to screening
  • More than 30 days of systemic immunosuppressive medication to include steroids in doses equivalent to or greater than 10 mg prednisone per day within 30 days prior to screening (inhaled corticosteroids are allowed)
  • Nursing mother or male partner of pregnant female.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Locteron ® PANEL AribavirinPANEL A: Locteron™ 480 µg dosed every 2 weeks in two subcutaneous injections (160 µg and 320 µg)
Locteron ® PANEL BribavirinPANEL B: Locteron™ 480 µg dosed every two weeks in single subcutaneous injections
PEG-Intron® PANEL BribavirinPEG-Intron® 1.5 µg/kg body weight weekly subcutaneous injection
PEG-Intron® PANEL AribavirinPEG-Intron® 1.5 µg/kg body weight weekly subcutaneous injection
Primary Outcome Measures
NameTimeMethod
Primary efficacy endpoint: EVR: the proportion of subjects in each arm that have at least a 2 log drop in HCV RNA from BaselineWeek 12
Secondary Outcome Measures
NameTimeMethod
the proportion of subjects in each arm demonstrating HCV RNA undetectable (< 10 IU/mL) after 12 weeks of randomized treatmentWeek 12

Trial Locations

Locations (15)

Tokuda Hospital

🇧🇬

Sofia, Bulgaria

Fundeni Clinical Institute

🇷🇴

Bucharest, Romania

UMHAT "St Maria"

🇧🇬

Varna, Bulgaria

Sourasky Medical Center

🇮🇱

Tel Aviv, Israel

"Victor Babes" Clinical Hospital Craiova

🇷🇴

Craiova, Romania

Holy Family Hospital Nazareth

🇮🇱

Nazareth, Israel

UMHAT "Alexandrovska"

🇧🇬

Sofia, Bulgaria

UMHAT "St Ivan Rilski"

🇧🇬

Sofia, Bulgaria

UMHAT "Queen Giovanna - ISUL" EAD

🇧🇬

Sofia, Bulgaria

Institute of Infectious Diseases

🇷🇴

Bucharest, Romania

Medical Institute Ministry of Interior

🇧🇬

Sofia, Bulgaria

Carmel Medical Center

🇮🇱

Haifa, Israel

Rabin Medical Center

🇮🇱

Petah-Tiqwa, Israel

Rebekah Ziv Medical Center Safed

🇮🇱

Zefat, Israel

Gastroenterology and Hepatology Institute

🇷🇴

Iasi, Romania

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