Pilot Study of Protein Profiling of the Human Epidermal Cells After Ultraviolet Irradiation

Early Phase 1

Completed

• Conditions: Proteomics
• Interventions: Procedure: UV Irradiation

• Registration Number: NCT00397436
• Lead Sponsor: University of Minnesota

Brief Summary

The main focus of this pilot study is to construct a fairly complete protein profile of the epidermis. This will allow us to study how the epidermis responds to external stressors such as ultra-violet. We hypothesize that by using the cutting edge technology, proteomics, we will be able to construct a fairly complete profile of different epidermal cells (keratinocytes, epidermal nerve fibers (ENFs), melanocytes, Langerhans cell,...etc) of normal and ultraviolet (UV) irradiated skin.

Detailed Description

We will investigate the changes of the epidermal cell proteome after UV irradiation to the forearms of healthy young male volunteers. Knowledge of specific molecular markers may provide knowledge of basic biological systems and possibly targeted therapy of inflammation.

By using combined proteomic methodologies we will be able to quantify protein changes at cytoplasmic and membrane protein levels of epidermal cells in normal vs. irradiated skins.

This study may allow us to obtain fairly comprehensive profile of membrane integrated and cytoplasmic proteins of epidermal cell populations which will facilitate our understand of biological responses to UV irradiation.

Study Timeline

• Study First Submitted: 2006-11-07
• Study First Submitted QC: 2006-11-08
• Study First Posted: 2006-11-09
• Study Start: 2007-02
• Primary Completion: 2011-11
• Study Completion: 2015-12
• Status Verified: 2016-05
• Last Update Submitted: 2016-05-13
• Last Update Posted: 2016-05-17

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 60

Inclusion Criteria

• Healthy Males age 18-30
• Caucasian
• No neurological disease

Exclusion Criteria

• Use of any medication that affects skin

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
1	UV Irradiation	Comparing irradiated skin to non irradiated skin.

Primary Outcome Measures

Name	Time	Method
Ability to quantify protein changes at cytoplasmic and membrane protein levels of epidermal cells in normal vs. irradiated skin	1 year

Secondary Outcome Measures

Name	Time	Method
Obtain a profile of membrane integrated and cytoplasmic proteins of epidermal cell populations which will facilitate our understanding of biological responses to UV irradiation.	1 year

Trial Locations

Locations (1)

University of Minnesota; 🇺🇸 Minneapolis, Minnesota, United States