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Multicountry Studies on the Effect of Positional Distribution of Fatty Acids at Triglyceride Backbone on Serum Lipids, Lipoprotein(a) and LDL-subclasses in Healthy Malaysian Volunteers

Not Applicable
Completed
Conditions
Cardiovascular Risk Factor
Registration Number
NCT02802904
Lead Sponsor
Malaysia Palm Oil Board
Brief Summary

Fats and oils are made up of \>90% triacylglycerol fat molecules which consist of a glycerol backbone to which 3 esterified fatty acids are attached. The positions of fatty acid attachment are referred to by stereospecific numbers, sn -1, -2 and -3. There is existing evidence to show that vegetable oils having unsaturated fatty acids in the sn-2 position with predominantly palmitic acid (16:0) or stearic acid (18:0) in the sn-1 and sn-3 positions of fat molecules do not raise serum cholesterol levels. These observations have come to be known as or explained by the "sn-2 hypothesis". New evidence have also emerged to show that saturated fatty acids (16:0, 18:0) in the sn-1 and -3 positions reduces fat deposition in a rat model. In this proposed study, the effects on the outcome measures investigated of three test fats \[namely palm olein IV64 (POP), virgin olive oil (OOO) and cocoa butter (POS)\], with oleic acid primarily at the sn-2 position but differing amounts of palmitic, stearic and oleic acids at the sn-1/sn-3 positions of the triglyceride molecule, are investigated

Detailed Description

To investigate the effects of different dietary fats with primarily oleic acid in the sn-2 position on the cardiovascular risk profile in healthy Malaysian volunteers

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
42
Inclusion Criteria

Not provided

Exclusion Criteria

Not provided

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Primary Outcome Measures
NameTimeMethod
Changes of Ratio of total cholesterol to HDL cholesterol (TC:HDL)week 0 (baseline) and week 4

analyzed enzymatically by Siemens Advia 2400 Chemistry Analyzer

Secondary Outcome Measures
NameTimeMethod
changes of serum Lp(a)week 0 (baseline) and week 4

analyzed enzymatically by Siemens Advia 2400 Chemistry Analyzer

changes of serum HDL cholesterolweek 0 (baseline) and week 4

analyzed enzymatically by Siemens Advia 2400 Chemistry Analyzer

changes of Blood pressureweek 0 (baseline) and week 4

measured by blood pressure meter

changes of serum non-esterified fatty acids (NEFA)week 0 (baseline) and week 4

analysed by ELISA kits

changes of Waist circumferenceweek 0 (baseline) and week 4
changes of serum LDL cholesterolweek 0 (baseline) and week 4

analyzed enzymatically by Siemens Advia 2400 Chemistry Analyzer

changes of serum LDL sub-fractionsweek 0 (baseline) and week 4

analysed with LIPOPRINT System

Changes of body mass index (BMI)week 0 (baseline) and week 4

measured by Tanita Segmental Body Fat Analysis

changes of serum Triacylglycerol (TAG)week 0 (baseline) and week 4

analyzed enzymatically by Siemens Advia 2400 Chemistry Analyzer

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