The Association of Transgelin-2, Metallothionein-2 and Ezrin With Asthma

Recruiting

• Conditions: Asthma
• Interventions: Diagnostic Test: No intervention

• Registration Number: NCT05496582
• Lead Sponsor: Shanghai University of Traditional Chinese Medicine

Brief Summary

Asthma is one of the most common chronic respiratory diseases. Previous study found a series of differentially expressed genes and proteins (transgelin-2, metallothionein-2, ezrin) in asthmatic rat. The aim of the study is to investigate the changes and associations of these gene with asthma in human.

Detailed Description

This study will investigate the changes and associations in asthma. In this project, the investigators will explore the changes of gene expression of transgelin-2, metallothionein-2 and ezrin in asthma and investigate their correlation with current diagnosis markers of asthma, including pulmonary function, fractional exhaled nitric oxide,eosinophil counts,immunoglobulin E, interleukin-5, etc.

Study Timeline

• Study First Submitted: 2022-08-09
• Study First Submitted QC: 2022-08-09
• Study First Posted: 2022-08-11
• Study Start: 2022-11-01
• Status Verified: 2024-04
• Last Update Submitted: 2024-05-06
• Last Update Posted: 2024-05-08
• Primary Completion: 2026-08
• Study Completion: 2026-12

Recruitment & Eligibility

• Status: RECRUITING
• Sex: All
• Target Recruitment: 100

Inclusion Criteria

1. Males or females, aged 14-75 years;
2. Patients with a definite diagnosis of bronchial asthma;
3. The diagnosis of asthma according to the Global Initiative for Asthma (GINA), with positive bronchodilator responsiveness (reversibility) test;
4. Healthy volunteers without respiratory symptoms such as cough, wheezing, chest tightness or dyspnea;
5. Agree with all procedures in this trial by signing a written informed consent form.

Exclusion Criteria

1. Patients with chronic obstructive pulmonary disease or other airway disease;
2. Complicated with other severe primary diseases (including hypertension, cancer, hyperthyroidism, bronchiectasia, cardiac insufficiency) and conditions that would prevent participation in the trial or put the participant at risk;
3. Women who are known to be pregnant or breastfeeding;
4. Not willing to participate;
5. Psychiatric history and neurological disorders can not work normally;
6. Other conditions that the researcher considers inappropriate to participate in this study.

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
Asthma	No intervention	Previously disgnosed asthma patients
Control	No intervention	Healthy participants

Primary Outcome Measures

Name	Time	Method
Difference in gene expression of transgelin-2, metallothionein-2 and ezrin between controls and asthmatic patients	Two year	PCR test of gene expression of transgelin-2, metallothionein-2 and ezrin between controls and asthmatic patients
Association of transgelin-2, metallothionein-2 and ezrin with asthma	Two year	Correlation analysis between gene expressions of transgelin-2, metallothionein-2, ezrin and current asthmatic tests (pulmonary function, fractional exhaled nitric oxide,immunoglobulin E, serum eosinophil counts, interleukin-5, etc)

Trial Locations

Locations (2)

Yueyang Hospital, Shanghai University of Traditional Chinese Medicine; 🇨🇳 Shanghai, Shanghai, China

Xiangya Hospital, Central South University; 🇨🇳 Changsha, Hunan, China