Expression of Toll-Like Receptors 2 and 4 in Asthmatic Patients.

Not Applicable

Completed

• Conditions: Asthma
• Interventions: Other: TLRs

• Registration Number: NCT02028637
• Lead Sponsor: Fundació Institut de Recerca de l'Hospital de la Santa Creu i Sant Pau

Brief Summary

Introduction: Recently it has involved the role of TLR (toll-like receptors) in the pathogenesis of asthma. TLRs are a family of proteins responsible for the recognition of Pathogen-Associated Molecular Patters (PAMPs). The possible role that TLRs play in IgE-dependent asthma (not eosinophilic) and its recognition as a new endotype asthma, could open new therapeutic expectations.

Objective: To analyze the expression of TLR 2 and 4 in monocytes/ macrophages and neutrophils in peripheral blood and induced sputum from asthmatic patients

Detailed Description

The investigators studied patients with asthma. All patients underwent the same day an induced sputum, pulmonary function studies, fractional exhaled nitric oxide, blood total IgE and skin test by prick technique. Asthmatic patients were classified according to the criteria of the GINA 2012. The cellular composition was analyzed by flow cytometry. Expression of TLR2 and TLR4 were analyzed using monoclonal antibodies conjugated with fluorochromes appropriate.

Study Timeline

• Study Start: 2009-07
• Primary Completion: 2013-09
• Study Completion: 2013-09
• Status Verified: 2014-01
• Study First Submitted: 2014-01-03
• Study First Submitted QC: 2014-01-04
• Last Update Submitted: 2014-01-04
• Study First Posted: 2014-01-07
• Last Update Posted: 2014-01-07

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 59

Inclusion Criteria

• Adults with persistent asthma (defined as per the Global INitiative for Asthma Management (GINA) criteria)

Exclusion Criteria

• If they had a respiratory tract infection or asthma exacerbation within 30 days prior to inclusion
• If they were receiving oral steroids or immunosuppressive treatments.

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
toll like receptor (TLRs)	TLRs	TLRs are a family of proteins responsible for the recognition of Pathogen-Associated Molecular Patters

Primary Outcome Measures

Name	Time	Method
The percentage of neutrophils expressing TLR4 in induced sputum decreases in asthma not eosinophilic	2 years	The percentage of neutrophils expressing TLR4 in induced sputum decreases in asthma not eosinophilic (IgEnodep).This could suggest that in this type of asthma the defense mechanism of neutrophils could be altered by decreasing expression of these receptors on their cell surface. This mechanism would still be defined. Probably the respiratory infections play an important role.

Trial Locations

Locations (1)

Hospital de la Santa Creu i Sant Pau; 🇪🇸 Barcelona, Spain