NCT03307278撤回不适用
Effects and Mechanism of House Dust Mite Induced Inflammasome Activation on Corticosteroid Resistance
适应症
干预措施
试验速览
- 阶段
- 不适用
- 状态
- 撤回
- 主要终点
- Steroid receptor phosphorylation by HDM
研究概览
简要总结
In this project, PBMCs will be isolated from HDM allergic and non allergic patient and then stimulated with HDM crude extract. The expression of inflammasome and the correlation of inflammasome activation and steroid resistance will be investigated.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 20 Years 至 70 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •HDM allergic and non allergic subjects
排除标准
- •Pregnant subject, cancer
研究组 & 干预措施
Steroid resistance in HDM allergic patients
Experimental
干预措施: House dust mite (Biological)
Steroid resistance in non HDM allergic subjects
Experimental
干预措施: House dust mite (Biological)
结局指标
主要结局
Steroid receptor phosphorylation by HDM
时间窗: 6 hours
The expression of steroid receptor will be detected by western blot and real-time PCR after HDM stimulation.
inflammasome activation by HDM
时间窗: 6 hours
The expression of NLRP3 in PBMCs will be measured by western blot after HDM stimulation.
次要结局
未报告次要终点
研究者
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