Influence of extraction and purification procedures on mRNA-based body fluid identificatio
Not Applicable
- Conditions
- o
- Registration Number
- JPRN-UMIN000023844
- Lead Sponsor
- Tokyo Medical and Dental University
- Brief Summary
RNA quality evaluation by RIN value showed that higher quality RNA was purified by the spin column method than by the magnetic bead method in blood, saliva and semen. On the other hand, since the magnetic bead method also extracts and purifies low-molecular-weight RNA, the real-time RT-PCR method had high sensitivity. When performing relative quantification by the dCt method, it was considered important to select an extraction/purification method and a control gene.
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Complete: follow-up complete
- Sex
- All
- Target Recruitment
- 4
Inclusion Criteria
Not provided
Exclusion Criteria
The person who does not take medicine.
Study & Design
- Study Type
- Observational
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method
- Secondary Outcome Measures
Name Time Method