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Nitrous Oxide as a Putative Novel Dual-Mechanism Treatment for Bipolar Disorder

Phase 2
Terminated
Conditions
Bipolar Disorder
Interventions
Registration Number
NCT02351869
Lead Sponsor
Sunnybrook Health Sciences Centre
Brief Summary

This study is a 7-day randomized, double-blind proof-of-concept pilot study of nitrous oxide vs. midazolam in 40 adults (20-60 years) with bipolar disorder (BD) (type I or II). Ongoing pharmacological and psychosocial treatments may continue, provided that they have not been initiated or significantly modified in the preceding 2 weeks. Participants' current treatment as prescribed by clinical psychiatrists will not be modified or interfered in this study. The study involves 3 visits. During study visit 1, participants will complete screening to ensure study eligibility. This will be done using interview measures. During study visit 2, participants will complete anthropomorphic measurements, measurement of endothelial function, screening blood work, ECGs, and an anaesthesia screener. During study visit 3, participants will receive the treatment (nitrous oxide or midazolam), complete an MRI scan, and complete interview measures and self-reports. There will be anthropomorphic measurements taken as well. The participant will be required to complete phone interviews and self-reports over the subsequent 7 days. There are 4 main predictions: 1. Nitrous oxide will significantly reduce depression symptoms vs. midazolam. 2. Nitrous oxide will significantly increase frontal cortical perfusion vs. midazolam. 3. Lower perfusion in frontal cortical regions at baseline will be associated with greater improvement in depression symptoms following nitrous oxide treatment. 4. Poorer endothelial function will be associated with greater improvement in depression symptoms following nitrous oxide treatment.

Detailed Description

Not available

Recruitment & Eligibility

Status
TERMINATED
Sex
All
Target Recruitment
25
Inclusion Criteria

English-speaking; age 20-60 years; BD-I or BD-II, current major depressive episode ≥4 weeks duration; MADRS≥22; taking ≥1 mood stabilizing medication/s (i.e. antimanic anticonvulsant, antipsychotic, and/or lithium).

Exclusion Criteria

New medications or changes in dosing, or ECT or TMS, in the preceding 2 weeks; MADRS item 10, > 4; YMRS≥12; acute significant suicidality; psychosis; substance abuse (past 3 months); active major medical conditions (hepatic, renal, respiratory, or cardio/cerebrovascular disease; diabetes; esophageal reflux; sleep apnea); B12 deficiency/disorders; pregnant; MRI contraindications; history of adverse anaesthetic reactions; anaesthesia class >2; scuba diving in preceding week.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Nitrous oxideNitrous OxideN2O-condition participants will inhale an initial mixture of 10% N2O in oxygen (O2) for 5 minutes, followed by 25% N2O in O2 for 20 minutes. N2O-condition participants will also receive 5ml intravenous saline concomitantly with 10% N2O, and again with 25% N2O.
MidazolamMidazolamInhaled room air plus intravenous midazolam bolus (total 2mg). Midazolam-condition participants will receive intravenous infusions of 0.5mg midazolam in 5ml saline (start of 1st inhalation epoch), followed by 1.5mg midazolam in 5ml saline (start of 2nd inhalation epoch).
Primary Outcome Measures
NameTimeMethod
Change in Montgomery-Asberg Depression Scale (MADRS) scoreAssessed at baseline, an average of 3 days later, again at up to 5 days after baseline on the day of drug administration, and participants will be followed for 7 days after the drug administration

Measures mood symptom severity, used to select patients and assess treatment efficacy. Although other time-points will be examined, 24h was selected as the primary outcome to minimize the impact of acute sedation and psychoactive effects.

Secondary Outcome Measures
NameTimeMethod
Young Mania Rating Scale (YMRS)Assessed at baseline, an average of 3 days later, again at up to 5 days after baseline on the day of drug administration, and participants will be followed for 7 days after the drug administration

Measures symptom severity

Blood PressureMeasured an average of 3 days post-baseline and again approximately every hour on the drug administration day
Visual Analogue ScaleAssessed on the drug administration day and followed for 7 days post-drug administration
HeightAssessed an average of 3 days after baseline
Biomarkers (B12 and nitric oxide (NO))Assessed an average of 3 days after baseline

B12 and nitric oxide (NO) will be examined as predictors of response owing to known associations with the mechanism of action of N2O.

The Clinician Administered Dissociative States Scale (CADSS)Assessed on the drug administration day and followed for 7 days post-drug administration
General Information Sheet (Demographics)Collected at baseline

Demographics

Hamilton Anxiety Rating Scale (HAM-A)Assessed on the drug administration day and followed for 7 days post-drug administration

Interview measure used to assess anxiety severity

Hamilton Depression Rating Scale (HDRS)Assessed on the drug administration day and followed for 7 days post-drug administration

Interview measure used to assess mood severity

Patient Rated Inventory of Side EffectsAssessed on the drug administration day and followed for 7 days post-drug administration

Self-report used to assess side effects

CANTAB Medication ListingAssessed an average of 3 days after baseline

Used to collect medications taken the day before and on the day of blood work

Structured Clinical Interview for DSM DisordersAssessed at baseline

Interview measure used to assess DSM disorders

Frontal Perfusion assessed using an MRI scanAssessed approximately 5 days after baseline and post-drug administration

Will be assessed using an MRI scan.

Heart RateMeasured an average of 3 days post-baseline and again approximately every hour on the drug administration day
Brief Psychiatric Rating ScaleAssessed on the drug administration day and followed for 7 days post-drug administration
WeightAssessed an average of 3 days after baseline
Beck Depression Inventory (BDI-II)Assessed on the drug administration day and followed for 7 days post-drug administration

Self-report measure of mood severity

Endothelial Function assessed via RH-PAT using the EndoPAT.Assessed an average of 3 days after baseline and lasts approximately 30 minutes

Will be assessed via RH-PAT using the EndoPAT.

Trial Locations

Locations (1)

Sunnybrook Health Sciences Centre

🇨🇦

Toronto, Ontario, Canada

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