Fish Oil T Cell Function
- Conditions
- Cognitive FunctionMuscle Strength
- Interventions
- Dietary Supplement: Olive OilDietary Supplement: Fish Oil
- Registration Number
- NCT02775084
- Lead Sponsor
- Texas A&M University
- Brief Summary
The purpose of the study is to examine the effect of dietary n-3 polyunsaturated fatty acid supplementation on subjects' pan CD4+ T cell function, cognition, and muscle function. Half of the participants will receive fish oil, while the other half will receive a placebo (olive oil).
- Detailed Description
Generalized inflammation has been consistently associated with aging and metabolic diseases, often characterized by reduced muscle and cognitive function. Although much of the aging-associated inflammation has been attributed to chronic activation of the innate host defense system, activated CD4+ T cells have been shown to contribute directly to the pathogenesis of several other inflammatory diseases. In humans, dietary n-3 polyunsaturated fatty acids (PUFA), particularly docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), have anti-inflammatory properties and reduce disease symptoms, in part, through suppression of CD4+ T cell activation. Therefore, the Researchers' overall hypothesis is that dietary supplementation with DHA and EPA in humans will ameliorate inflammatory symptoms, in part, by suppressing CD4+ T cell activation, positively affecting muscle and cognitive function.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 13
Not provided
Not provided
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Arm && Interventions
Group Intervention Description Olive Oil Olive Oil 8 grams olive oil Healthy Fish Oil Fish Oil 8 grams fish oil
- Primary Outcome Measures
Name Time Method Change in function of of lipid rafts in immune cell membranes due to dietary polyunsaturated fatty acids supplementation one time on baseline, day 21 and day 42 one blood draw on each study visit
- Secondary Outcome Measures
Name Time Method Fatty acid metabolism one time on baseline, day 21 and day 42 One blood draw on each study visit
Body Composition 15 minutes on baseline, day 21 and day 42 Body composition as measured by Dual-Energy X-ray Absorptiometry
Diet recall on baseline and change from day 21 and day 42 The subject is asked to recall in detail all the food and drink consumed during the 24 hours prior to the test day.
Group differences in attention and executive functions as measured by Stroop Color-Word Test (SCWT) Postabsorptive state during 1 hours and change after feeding on baseline and change from day 21 and day 42 A word page with words printed in black ink, a color page with blocks printed in color, and a color-word page where the color and the word do not match. The examinee reads the words or names the ink colors as quickly as possible within a time limit. Measures selective attention and inhibitory control. The total time in seconds was reported for each trial.
Group differences in learning and memory as measured by Digit Span Postabsorptive state during 1 hours and change after feeding on baseline and change from day 21 and day 42 Recall of numbers in the same order (Digit Forward) and in reverse order (Digit Backward). Measures auditory attention and verbal working memory.
Group differences in state of mood as measured by the Hospital Anxiety and Depression Scale (HADS Postabsorptive state during 1 hours and change after feeding on baseline and change from day 21 and day 42 A fourteen item self-assessment scale. Seven of the items related to anxiety and seven relate to depression. Each item on the questionnaire is scored from 0-3 and this means that a person can score between 0 (no symptoms) and 21 (severe symptoms) for either anxiety or depression.
Executive Function-Adolescent/Adult Sensory Profile (ASP) Postabsorptive state during 1 hours and change after feeding on baseline and change from day 21 and day 42 A standardized self-questionnaire that generates an individualized profile of sensory processing across four quadrants: low registration, sensation seeking, sensory sensitivity, and sensation avoiding.
Group differences in attention and executive functions as measured by Brief-A Postabsorptive state during 1 hours and change after feeding on baseline and change from day 21 and day 42 A standardized self-report that captures views of an adult's executive functions or self-regulation in his or her everyday environment.
Group differences in attention and executive functions as measured by Trail Making Test (TMT) Postabsorptive state during 1 hours and change after feeding on baseline and change from day 21 and day 42 In Part A, the examinee is instructed to connect a set of 25 circles with numbers as quickly as possible while maintaining accuracy. In Part B, the examinee is instructed to connect a set of 25 circles, alternating between numbers and letters, as quickly as possible while maintaining accuracy. Measures attentional resources and is a measure of the frontal lobe "executive" functions of visual search, set-switching and mental flexibility. The total time in seconds was reported for each measure.
Group differences in overall cognitive abilities as measured by Montreal Cognitive Assessment (MoCA) Postabsorptive state during 1 hours and change after feeding on baseline and change from day 21 and day 42 Assesses several cognitive domains and is used for the screening of mild cognitive impairment. Total scores range from 0-30 with lower scores indicating decreased functioning.
Skeletal muscle strength of leg 30 minutes on baseline, day 21 and day 42 Measurement of muscle strength of leg using kin-com machine
Skeletal muscle strength of hand 30 minutes on baseline, day 21 and day 42 Measurement of hand grip strength
Trial Locations
- Locations (1)
Texas A&M University-CTRAL
🇺🇸College Station, Texas, United States