Analysis of the pulmonary microbiome in patients with respiratory failure

• Conditions: A41.9; J80.01; Sepsis, unspecified

• Registration Number: DRKS00018799
• Lead Sponsor: niveristätsklinikum Heidelberg Klinik für Anästhesiologie

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Study Start: 2019-11-18
• Last Update Posted: 19 August 2024

Recruitment & Eligibility

• Status: Pending
• Sex: All
• Target Recruitment: 60

Inclusion Criteria

Healthy volunteers
Included are lung healthy volunteers who, in the period under investigation a thoracoabdominal oesophageal resection and the procedure is performed of this study. A total of 10 lung healthy volunteers are expected to be enrolled in the study will be included. Inclusion takes place in chronological order. The following must be included the following criteria must be met:
- written declaration of consent by the study participant
- Age >18 years
- no acute or chronic inflammatory lung disease

Patients with acute respiratory failure
This includes all patients who have to be intubated and ventilated at the Surgical Clinic of Heidelberg University Hospital due to acute respiratory failure during the examination period. A total of 50 patients will be included in the study. Inclusion will take place in chronological order. The following criteria must be fulfilled:
- Acute respiratory failure
- Invasive ventilation via endotracheal tube or tracheostoma
- written declaration of consent by the study participant or his legally appointed representative
- Age >18 years

Exclusion Criteria

- Refusal to participate in the study
- pregnancy
- Age <18 years

Study & Design

• Study Type: observational
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Examination of pulmonary microbiome in ARDS (directly after onset and intubation) compared to lung healthy patients by Next Generation Sequencing

Secondary Outcome Measures

Name	Time	Method
- Investigation of changes in the microbiome in correlation with clinically relevant complications.<br>- Evaluation of the prognostic significance of microbial analysis.<br>- Correlation between pathogen diagnosis via NGS (Next Generation Sequencing) and culture based.