Study Of Adipose Tissue Function and Weight Development Over Time
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Obesity
- Sponsor
- Karolinska Institutet
- Enrollment
- 105
- Locations
- 1
- Primary Endpoint
- Weight development and correlation to lipogenesis
- Status
- Completed
- Last Updated
- 9 years ago
Overview
Brief Summary
The investigators will study the influence of fat cell size/number and adipose function on weight development over very long time periods (years). By comparing fat biopsies obtained at baseline and after >7 years, the investigators will determine the association between adipose morphology/function and changes in weight or development of cardiometabolic complications (e.g. insulin resistance, type 2 diabetes, dyslipidemia and hypertension).
Detailed Description
The investigators will ask all subjects that have previously been examined at the investigators laboratory (from 1992 and forward) in studies of adipose tissue function if they are interested to participate in a new study. The minimal follow up time is 7 years. Saved blood samples and samples from old fat biopsies will be analyzed together with samples from new examinations. The investigators plan to examine the subjects in the same way as performed at the baseline examinations. This includes estimation of total body fat using bioimpedance, venous blood samples for determination of insulin, glucose and lipids, subcutaneous fat biopsies with determination of fat cell size/number, lipolysis/lipogenesis as well as analysis of adipokine secretion. In selected patients, baseline examinations included DEXA-measurement (dual energy x-ray absorptiometry) for determination of body fat distribution as well as hyperinsulinemic euglycemic clamp. In these cases, the same type of examinations will be repeated at their follow up visits.
Investigators
Mikael Ryden
MD, PhD, Professor
Karolinska Institutet
Eligibility Criteria
Inclusion Criteria
- •Participated in previous examination at our lab at least 7 years ago.
- •No blood samples or adipose samples saved from last examination
Exclusion Criteria
- •Decline to participate after invitation.
- •Serious illness or mental disorder.
- •Warfarin or other new oral anticoagulant that increase risk of bleeding.
Outcomes
Primary Outcomes
Weight development and correlation to lipogenesis
Time Frame: 2014 to 2024, up to 10 years
Recruitment of these subjects will continue until 2024 with a minimum follow up time of 7 years. Weight will be assessed with a regular scale and lipogenesis will be measured according to previously published standardized protocol after isolated fat cells have been stimulated with insulin.
Weight development over time and correlation to adipose morphology
Time Frame: from 2014 up to 2024, up to 10 years
Recruitment of these subjects will continue until 2024 with a minimum follow up time of 7 years (84 months). Weight will be assessed with a regular scale and adipose morphology will be determined by measurement of fat cell size and adjustment for body weight.
Weight development and correlation to lipolysis
Time Frame: 2014 to 2024, up to 10 years
Recruitment of these subjects will continue until 2024 with a minimum follow up time of 7 years. Weight will be assessed with a regular scale. Fat cells will be isolated and assessment of lipolysis will be made with previously published standardized protocols.
Secondary Outcomes
- adipose morphology and development of fat mass(2014 to 2024, up to 10 years)
- adipose morphology and changes in fat mass(2014 to 2024, up to 10 years)
- adipose tissue morphology development and insulin sensitivity(2014 to 2024, up to 10 years)