Analysis of Pregnancy-Associated Plasma Protein-A and Interleukin-6 Gene Polymorphisms in Patients With Periodontitis

Completed

• Conditions: Periodontitis; Polymorphism, Restriction Fragment Length
• Interventions: Genetic: genetic polymorphism analysis

• Registration Number: NCT06540755
• Lead Sponsor: Marmara University

Brief Summary

The aim of this study is to evaluate the relationship between pregnancy associated plasma protein-A (PAPP-A) and interleukin-6 (IL-6) gene polymorphisms and stage 3 grade B and C (S3GBC) periodontitis, and to compare periodontitis patients with healthy individuals.

Detailed Description

A total of 226 systemically healthy subjects, consisted of 112 S3GBC periodontitis patients (P group) and 114 periodontally healthy individuals (H group) were included in our study. Clinical and demographic data were recorded. Genomic deoxyribonucleic acid (DNA) molecules were obtained from peripheral blood. Genotype for IL-6 rs1800795 and PAPP-A rs7020782 polymorphisms were determined using real-time polymerase chain reaction with allele-specific probes. The genotypic or allelic distributions of PAPP-A and IL-6 gene polymorphisms were similar between groups (p\>0.05). Our finding suggests that there is no relationship between PAPP-A and IL-6 gene polymorphismS and S3GBC periodontitis, furthermore no difference was detected between periodontitis patients and healthy individuals regarding this gene polymorphism.

Study Timeline

• Study Start: 2022-12-02
• Primary Completion: 2023-01-26
• Study Completion: 2024-01-28
• Status Verified: 2024-08
• Study First Submitted: 2024-08-02
• Study First Submitted QC: 2024-08-02
• Last Update Submitted: 2024-08-02
• Study First Posted: 2024-08-06
• Last Update Posted: 2024-08-06

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 226

Inclusion Criteria

Participants were eligible for inclusion if they were; systemically healthy non-smokers had not undergone periodontal treatment in the past 6 months had not used antibiotics or anti-inflammatory drugs in the preceding 3 months had not used any medication that could affect periodontal tissues in the past 6 months were 18 years of age or older were not pregnant, and were not lactating.

Exclusion Criteria

The samples taken cannot be stored under appropriate conditions and are damaged.

The onset of a systemic disease in a systemically healthy individual.

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
P Group	genetic polymorphism analysis	stage 3 grade B and C periodontitis patients
H Group	genetic polymorphism analysis	periodontally healthy individuals

Primary Outcome Measures

Name	Time	Method
The genotypic or allelic distributions of PAPP-A and IL-6 gene polymorphisms	baseline	they were similar between groups

Trial Locations

Locations (1)

Marmara University Dental Faculty Periodontology Departmen; 🇹🇷 İstanbul, Turkey