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Fosfomycin Versus Meropenem or Ceftriaxone in Bacteriemic Infections Caused by Multidrug Resistance in E.Coli

Phase 3
Completed
Conditions
Infection Due to ESBL Escherichia Coli
Interventions
Registration Number
NCT02142751
Lead Sponsor
Fundación Pública Andaluza para la gestión de la Investigación en Sevilla
Brief Summary

Enterobacterieaceae (and specially Escherichia coli) showing resistance due to multidrug-resistant Escherichia coli, plasmid mediated AmpC or quinolone resistance caused by chromosomal mechanisms have spread worldwide during the last decades. This is important because many of these isolates are also resistant to other first-line agents such as fluoroquinolones or aminoglycosides, leaving few available options for therapy, and this condition is associated with increased morbidity- mortality and length of hospital stay. While carbapenems are considered the drugs of choice for multidrug-resistant Escherichia coli and AmpC producers, recent data suggests that certain alternatives may be suitable for some types of infections.

At the present time, finding therapeutic alternatives to carbapenems and cephalosporins for the treatment of invasive infections due to multidrug-resistant Escherichia coli is critical. Fosfomycin was discovered more than 40 years ago but was not investigated according to present standards, and thus is not used in clinical practice except in desperate situations. It is one of the so-considered neglected antibiotics with high potential interest for the future.

With the aim of demonstrate the clinical non-inferiority of intravenous fosfomycin compared to meropenem or ceftriaxone in the treatment of bacteraemic urinary tract infections caused by multidrug-resistant Escherichia coli . The investigators propose a "real practise" randomised, controlled, multicentre phase III clinical trial to compare the clinical and microbiological efficacy and safety of intravenous fosfomycin (4 grammes every 6 hours) with meropenem (1 gramme every 8 hours) or ceftriaxone (1 gramme every 24 hours) as targeted therapy of the previously specified infection; change to oral therapy according to predefined options is allowed in both arms after 5 days. Follow-up for the study is planned up to 60 days.

Detailed Description

The FOREST study is a phase 3, randomised, controlled, multicentric, open-label clinical trial to prove the noninferiority of fosfomycin versus meropenem in the targeted treatment of bacteraemic UTI due to ESBL-EC, designed as a real practice trial. It is a non-commercial, investigator-driven clinical study funded through a public competitive call by Instituto de Salud Carlos III, Spanish Ministry of Economy (PI13/01282).

The study is coordinated by investigators from Hospital Universitario Virgen Macarena in Seville, Spain; the sponsorship is performed by Fundación Pública Andaluza para la Gestión de la Investigación en Salud de Sevilla (FISEVI), of which the sponsor-scientific responsibilities are delegated to the CTU (Clinical Trial Unit-Hospital Universitario Virgen del Rocío, Seville, Spain). All participating patients or their relatives must give written informed consent before any study procedures occur, including the withdrawal of biological samples for the study.

The hypothesis to test is that intravenous fosfomycin is not inferior to meropenem for the targeted treatment of bacteraemic UTI caused by ESBL-EC in terms of efficacy.

The primary objective of the study is to demonstrate that intravenous fosfomycin is not inferior to meropenem for reaching clinical and microbiological cure 5-7 days after the completion of treatment.

Secondary objectives include comparing the early clinical and microbiological response, 30-day mortality, hospital stay, recurrence rate, safety and impact on intestinal colonisation by MDR Gram-negative bacilli, evaluation of the rate of resistance development to fosfomycin and blood level concentration of fosfomycin.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
161
Inclusion Criteria
  • ≥18 years old hospitalized patients
  • Negative pregnancy test in fertile women
  • Episode of clinically-significant monomicrobial urinary BSI due to multidrug-resistant E.coli susceptible to fosfomycin and meropenem or ceftriaxone
  • Urinary sepsis with multidrug resistant E. coli isolation from the blood cultures, requires at least one clinical criteria and one of the following urinalysis criteria:

Clinical criteria

  • UTI symptoms (dysuriac, urgency, suprapubic pain or pollakiuria)
  • Lumbar back pain
  • Cost-vertebral angle tenderness
  • Altered mental status in people up to 70 years old
  • Intermittent or permanent indwelling foley catheter (or withdrawal during 24 hours previous) even without urinary symptoms urinalysis criteria
  • Urine dipstick test positive for either nitrites or leukocyte esterase
  • Positive urine culture - Signed informed consent form (ICF) executed prior to protocol screening assessments
Exclusion Criteria
  • Polymicrobial bacteremia
  • No drainage of renal abscess or obstructive uropathy unresolved
  • Pregnant or careening women
  • Haematogenous infection
  • Other concomitant infection
  • Renal transplantation recipients
  • Polycystic kidney
  • Hypersensitivity and/or intolerance to meropenem or fosfomycin or ceftriaxone
  • Palliative care or life expectance < 90 days
  • Septic shock at time of randomization
  • New York Heart Association (NYHA) functional Class IV, hepatic cirrhosis or renal impairment receiving dialysis
  • Active empiric treatment >72 hours
  • Late randomization >24 hours after multidrug resistant.coli blood culture´s identification
  • Participation in other clinical trial with active treatment

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Meropenem intravenousMeropenem intravenous1g every 8 hours (15-30 min infusion)
Fosfomycin sodium intravenousFosfomycin sodium intravenous4g every 6 hours iv (60 min infusion)
Ceftriaxone intravenousCeftriaxone intravenous1g every 24h (2-4 min)
Primary Outcome Measures
NameTimeMethod
Clinical and microbiological cure rateDay 5-7 after end of treatment (test of cure)

Clinical Cure: Complete resolution of infection symptoms (bacteremia and/or urinary tract infection-UTI-), present at the day on which blood culture was drawn.

Microbiological cure: Negative blood culture at day 5-7 after end of treatment. Besides this, if UTI was confirmed with a positive urine culture with the same microorganism than the blood culture, this culture should become negative.

Secondary Outcome Measures
NameTimeMethod
Early clinical responseAfter 5 -7 days of complete treatment (from the first day of study drugs administration)

The infection was completely resolved after 5-7 days of complete treatment

Early microbiological responsewithin the first 5 days after treatment started

Cultures are negative

Safety of intravenous antibiotic administration in this indicationTo the last visit, at 60 plus-minus 10 days (from the first day of study drugs administration)

Gathering any related adverse event from the informed consent form signature to the end of follow-up.

Length of hospital stayAt day 30 of follow-up

It is defined as the time from admission to hospital discharge

Recurrences (relapse and reinfection) rateTo the last visit, at 60 plus-minus 10 days (from the first day of study drugs administration)

Relapse: new symptoms of UTI in patient with previously considered as clinical or microbiological cured in the visit of day 5-7 plus positive urine or blood cultures with the same microorganism isolated than the initial culture.

Re-infection: same definition but with different strain in the culture results.

MortalityAt day 30 of follow-up

Death for any reason.

Fosfomycin steady-state plasma concentrationAt 3 days after treatment started

Therapeutic drug monitoring of fosfomycin, basic pharmacokinetic parameters will be determined.

Microbiota impact of study treatment bacilliScreening, day 5-7, day 12

Study treatment impact in the gut colonization of MDRGNB (Multi drug resistant Gram negative bacilli)

Safety of intravenous fosfomycin in this indicationTo the last visit, at 60 plus-minus 10 days (from the first day of study drugs administration)

Gathering any related adverse event from the informed consent form signature to the end of follow-up.

Emergence of resistant clinical isolates of Escherichia coli to fosfomycin and meropenemparticipants will be followed for the duration of fosfomycin, an expected average of 14 days

Frequency of strains that develop resistance and detection of resistance mechanisms in fosfomycin treatment arm.

Trial Locations

Locations (22)

Hospital Arnau de Vilanova

🇪🇸

Vilanova, Lleida, Spain

Hospital Royo Villanova

🇪🇸

Zaragoza, Spain

Hospital Universitario 12 de Octubre

🇪🇸

Madrid, Spain

Hospital Marqués de Valdecilla

🇪🇸

Santander, Spain

Hospital Son Espases

🇪🇸

Palma de Mallorca, Spain

Hospital Universitario Central de Asturias

🇪🇸

Oviedo, Spain

Hospital Universitario Virgen Macarena

🇪🇸

Sevilla, Spain

Hospital Mutua de Terrassa

🇪🇸

Terrassa, Barcelona, Spain

Hospital Universitario de Gran Canaria Dr. Negrín

🇪🇸

Las Palmas De Gran Canaria, Gran Canarias, Spain

Hospital de la Santa Creu i San Pau

🇪🇸

Barcelona, Spain

Hospital Universitario de Canarias

🇪🇸

La Laguna, Tenerife, Spain

Hospital Marina Baixa

🇪🇸

Alicante, Spain

Hospital Clínico Universitario Virgen de la Arrixaca

🇪🇸

El Palmar, Murcia, Spain

Hospital Vall d'Hebron

🇪🇸

Barcelona, Spain

Hospital General Universitario de Alicante

🇪🇸

Alicante, Spain

Hospital Parc Salud Mar

🇪🇸

Barcelona, Spain

Hospital Universitario de Bellvitge

🇪🇸

Barcelona, Spain

Hospital Universitario Reina Sofía

🇪🇸

Córdoba, Spain

Hospital Universitario de Burgos

🇪🇸

Burgos, Spain

Hospital Ramón y Cajal

🇪🇸

Madrid, Spain

Hospital de Cruces

🇪🇸

Bilbao, Spain

Hospital Universitario y Politécnico La Fe

🇪🇸

Valencia, Spain

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