Beta-lactoglobulin, Immobilisation and Muscle Protein Synthesis

Not Applicable

• Conditions: Muscle Atrophy
• Interventions: Dietary Supplement: Carbohydrate; Dietary Supplement: Beta-lactoglobulin

• Registration Number: NCT05496452
• Lead Sponsor: King's College London

Brief Summary

The objectives of this study are to:

1. Establish whether combined β-lactoglobulin supplementation and resistance training for 1 week prior to 5 days of limb immobilisation will attenuate the decrease in integrated free-living rates of MPS during short-term muscle disuse.

2. Establish whether combined β-lactoglobulin supplementation and resistance training for 1 week prior to limb immobilisation will attenuate the decrease in muscle mass and strength during short-term muscle disuse.

Detailed Description

The objectives of this study are to:

1. Establish whether combined β-lactoglobulin supplementation and resistance training for 1 week prior to 5 days of limb immobilisation will attenuate the decrease in integrated free-living rates of MPS during short-term muscle disuse.

2. Establish whether combined β-lactoglobulin supplementation and resistance training for 1 week prior to limb immobilisation will attenuate the decrease in muscle mass and strength during short-term muscle disuse.

Hypotheses

1. Combining β-lactoglobulin and resistance training for 1 wk prior to 5 d of limb immobilisation will attenuate the decrease in integrated muscle protein synthesis, muscle fibre CSA, quadriceps muscle strength, and leg lean mass during muscle disuse.

2. Combining β-lactoglobulin and resistance training for 1 wk prior to 5 d of limb immobilisation will attenuate the decrease in muscle fibre CSA, quadriceps muscle strength, and leg lean mass during muscle disuse.

Study Timeline

• Status Verified: 2022-08
• Study First Submitted: 2022-08-09
• Study First Submitted QC: 2022-08-09
• Study First Posted: 2022-08-11
• Study Start: 2023-04-01
• Last Update Submitted: 2023-04-24
• Last Update Posted: 2023-04-26
• Primary Completion: 2024-03-01
• Study Completion: 2024-08-01

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: All
• Target Recruitment: 24

Inclusion Criteria

• Male and female
• Aged 18-45 years
• Healthy
• Physically active (≥150-300 minutes of moderate-intensity aerobic physical activity per week OR 75-150 minutes of vigorous-intensity aerobic physical activity per week)
• Eumenorrheic and not taking any hormonal birth control (females)

Exclusion Criteria

• Dairy allergy or intolerance
• Lower limb injury or surgery in the last 6 months,
• Lower limb osteoarthritis or other musculoskeletal disorder
• A musculoskeletal or blood blotting disorder
• An allergy to local anaesthetic
• Currently pregnant
• Current use of blood thinning medications
• Volunteers that take part in structured resistance exercise training
• Taking supplements considered to be anabolic to skeletal muscle (protein supplements, creatine, or omega-3 supplements)
• Consuming more than 1.2 grams of protein per kilogram of body mass per day in their habitual diet.
• Volunteers involved in other studies at the time of enrolment
• Volunteers who have taken part in a tracer study in the past 18 months
• If without an understanding of verbal or written English
• Volunteers with a history of eating disorders

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Carbohydrate	Carbohydrate	Daily supplementation over 12 day period. Energy matched control.
Beta-lactoglobulin	Beta-lactoglobulin	Daily supplementation over 12 day period. A nutritional supplement.

Primary Outcome Measures

Name	Time	Method
Muscle protein synthesis	15 days	The synthesis of amino acids into new muscle protein.

Secondary Outcome Measures

Name	Time	Method
Muscle mass	15 days	Lean body mass will be measured using Dual Energy Xray Absorptiometry.

Trial Locations

Locations (1)

King's College London; 🇬🇧 London, United Kingdom