Genetic Evolution of Arboviruses in New Caledonia Between 1995 and 2024 and Impact of Wolbachia
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 600
- 试验地点
- 1
- 主要终点
- arbovirus genome sequencing
研究概览
简要总结
Arboviruses, diseases transmitted to humans by the bite of an insect vector, are a major public health problem, especially in tropical and sub-tropical countries. A promising strategy aimed at blocking the circulation of arboviruses is to release Aedes aegypti mosquitoes carrying the endosymbiotic bacterium Wolbachia. In 2019, the Wolbachia strategy was implemented in Nouméa as part of the World Mosquito Program. This intervention will modify the epidemiological profile of arboviruses in New Caledonia.
Epidemiological surveillance of arboviruses requires molecular characterization of the virus contained in the serum obtained from the blood collected from patients. This molecular characterization by RNA isolation techniques, RT-qPCR monitoring and sequencing allows the construction of phylogenetic trees.
In the context of the implementation of the World Mosquito Program in Nouméa, the investigators plan to follow the molecular evolution of arboviruses, over the period preceding the releases of mosquitoes carrying Wolbachia (from 1995 to 2019) then over a period of 5 years. following the releases.
At the same time, the virus can be isolated by cell culture techniques and in vitro infections, allowing its study in vitro in cells or in vivo in mosquitoes. This study allows us to measure the impact of the Wolbachia strategy on the evolution of the virus's ability to replicate in cells in the presence of Wolbachia and to be transmitted by the mosquito.
详细描述
Collection of blood samples from patients with arbovirus infection
This study is a non-interventional study with retrospective part.
This study will improve :
- the assessment of the molecular evolution of arboviruses in the period preceding the implementation of the Wolbachia strategy
- the knowledge of the impact of Wolbachia on the diversity and molecular evolution of arboviruses
- the understanding of arbovirus transmission and replication mechanisms in the presence of Wolbachia
研究设计
- 研究类型
- Observational
入排标准
- 年龄范围
- 2 Years 至 —（Child, Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Appearance of clinical signs suggestive of an infection with an arbovirus in the 7 days preceding the blood sample,
- •Diagnostic confirmation by RT-qPCR of mono-infection with dengue virus or another arbovirus.
- •Patients or their representative (s) informed of the possible secondary use for research purposes of the samples initially taken for diagnostic purposes and their derivatives, and who have not since expressed their opposition to the secondary use of the samples diagnostics or their derivatives for research,
排除标准
- •Documented opposition from a patient or his representative (s)
- •Previously established viral load too low.
- •Sample obtained from a sample taken more than 7 days after the appearance of clinical signs of infection.
结局指标
主要结局
arbovirus genome sequencing
时间窗: 5 years
Whole genome sequencing of virus extracted of blood samples from patients collected from 1995 to 2024
genetic evolution of arbovirus strains
时间窗: 5 years
bioinformatical analysis of the genome of the strains of arbovirus contained in serum samples collected from 1995 to 2024
次要结局
- measurement of the impact of Wolbachia on the virus genetic evolution(5 years)
- measurement of the ability of these viruses to replicate in the presence of Wolbachia(5 years)