Virological and Immunological Determinants of Arbovirus Infection in New Caledonia
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 250
- 试验地点
- 1
- 主要终点
- Measurement of vector competence parameters of Aedes aegypti (carriers or not of Wolbachia) for arboviruses by vector competence experiments using deported blood meals.
研究概览
简要总结
Arboviruses, diseases transmitted to humans by the bite of an insect vector, are a major public health problem, particularly in tropical and sub-tropical countries. In New Caledonia, dengue epidemics are recurrent and may be associated with the co-circulation of other arboviruses such as Zika or chikungunya.
The virological determinants which condition the occurrence of these epidemics may be linked to an increased vectorial competence of the vector mosquito Aedes aegypti for a particular viral isolate. In fact, the Aedes aegypti mosquito is infected by making a blood meal on a person infected with an arbovirus. The virus infects its digestive tract, then spreads throughout the mosquito's body until it reaches its salivary glands. The virus is then present in the saliva and will be injected into the human host during a new blood meal. Some viral variants are best transmitted by Aedes aegypti. In general, the study of this vectorial competence is carried out by experiments in the laboratory during which an artificial blood meal composed of mammalian blood (human, rabbit, etc.) is mixed with a viral stock. Carrying out deported blood meals during which blood collected from patients infected with an arbovirus is used to gorge mosquitoes makes it possible to place oneself in experimental conditions as close as possible to the natural cycle of transmission of arboviruses.
In the human host, cells of the myeloid lineage present in the peripheral blood constitute preferred targets of replication for arboviruses. At the same time, the peripheral blood cells of patients are activated in response to infection and secrete many soluble factors released into the blood of patients. The study of blood samples from patients infected with arboviruses is therefore of prime importance for understanding both the replicative mechanisms of arboviruses but also the immune response they induce.
详细描述
Collection of blood samples from adult patients with clinical signs suggestive of arbovirus
This study is a interventional study that present minimal risks and constraints.
This study will improve :
- the reliability of the results of vector competence experiments
- the understanding of the mechanisms of infection and replication of arboviruses
- the knowledge of the immune mechanisms involved in the response to infection by an arbovirus
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Major patient
- •Patient with clinical signs suggestive of an arbovirus infection:
- •Fever And at least 2 clinical signs suggestive of arbovirus among which myalgia, retro-orbital pain, drop in platelets (if data available), digestive symptoms, etc.
- •Appearance of clinical signs suggestive of an arbovirus infection in the 7 days preceding the blood sample.
- •Patient informed of the performance of the research, the collection of data and biological samples
- •Patient who has given their consent to participate in the research by authorizing the collection of data, the collection of an additional blood sample and the use of this sample
排除标准
- •Patient suffering from another identified virosis, an inflammatory disease or taking anti-inflammatory drugs
- •Pregnant patient
- •Patient returning from a trip within 15 days before the onset of the signs,
- •Patient for whom the blood sample is incomplete or could not be performed
- •Patient's condition, which, in the doctor's opinion, is incompatible with the additional sample required by the study
- •Patient not having consented to participate in the research
结局指标
主要结局
Measurement of vector competence parameters of Aedes aegypti (carriers or not of Wolbachia) for arboviruses by vector competence experiments using deported blood meals.
时间窗: 5 years
Rate of infection of Aedes aegypti (carriers or not of Wolbachia) for arboviruses
次要结局
- Molecular characterization of arborviruses strains included in the blood of patients and which may be diffused by mosquitoes carrying Wolbachia or not during deported blood meals(5 years)
- Molecular characterization of arborviruses strains contained in mosquitoes present in patients' homes(5 years)