Metabolome and Microbiomic in Fecal Samples

Completed

• Conditions: Infant

• Registration Number: NCT00938028
• Lead Sponsor: Massachusetts General Hospital

Brief Summary

The purpose of this study is to evaluate the effects of storage and handling on fecal samples collected for metabolomic, proteomic and microbiomic and analysis.

Detailed Description

Background

Human fecal collection for clinical or research studies usually occurs at the residence of the subject, an environment not equipped with measures to accommodate proper preparation and storage of samples for microbiome and metabolome analysis.

Aims

To evaluate the effects of storage and handling on fecal samples collected for metabolomic, proteomic and microbiomic and analysis.

Methods

Fecal samples will be collected within several minutes of voiding from healthy infants at the time of diaper change at day-care center.

Samples will be divided into 7 tubes, and subjected to different storage temperatures, before preforming microbial analysis by DNA sequencing, and metabolome analysis by H1NMR spectroscoty.

Study Timeline

• Study Start: 2009-04
• Primary Completion: 2009-05
• Study Completion: 2009-05
• Status Verified: 2009-07
• Study First Submitted: 2009-07-09
• Study First Submitted QC: 2009-07-10
• Study First Posted: 2009-07-13
• Last Update Submitted: 2009-07-17
• Last Update Posted: 2009-07-21

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 4

Inclusion Criteria

• healthy
• age 12-20 months

Exclusion Criteria

Not provided

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Microbiome and metabolome yield	one month

Trial Locations

Locations (1)

MassGeneral for Children; 🇺🇸 Boston, Massachusetts, United States