The Implementation of Novel 'Heavy' Water Techniques for Determining Modulation of Muscle Protein, DNA and Lipid Synthesis Due to Ageing

Not Applicable

Completed

• Conditions: Healthy
• Interventions: Behavioral: Resistance Exercise

• Registration Number: NCT02152839
• Lead Sponsor: University of Nottingham

Brief Summary

Maintenance of body's skeletal muscle is key to a healthy older age. However, as we age we lose on average 1-2% of our muscle each year. In order to slow this loss we need to understand fully the mechanisms regulating muscle mass with ageing. In this project we aim to determine these mechanisms using a new novel technique of 'heavy' water ingestion, which will allow us to measure multiple aspects of skeletal muscle mass control during normal everyday activities in young and old individuals over a period of 6 weeks. We will also determine the influence of resistance exercise training during this period in offsetting declines in muscle with ageing using this method

Detailed Description

Not available

Study Timeline

• Study Start: 2013-05
• Study First Submitted: 2014-05-29
• Study First Submitted QC: 2014-05-30
• Study First Posted: 2014-06-02
• Primary Completion: 2014-07
• Status Verified: 2015-07
• Last Update Submitted: 2015-07-30
• Last Update Posted: 2015-07-31

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 20

Inclusion Criteria

• Young (18-30y) and old men (65-75y) who are generally healthy and recreationally active

Exclusion Criteria

• Active cardiovascular disease
• Cerebrovascular disease including previous stroke, aneurysm (large vessel or intracranial)
• Respiratory disease including pulmonary hypertension or COPD
• Hyper/ hypo parathyroidism, hyper/ hypothyroidism, Cushing's disease, diabetes
• Active inflammatory bowel disease
• Renal disease
• Malignancy
• Recent steroid treatment (within 6 mo), or hormone replacement therapy
• Clotting dysfunction
• Musculoskeletal or neurological disorders
• Any disease requiring long-term drug prescriptions, including statins

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Old Unilateral Exercise	Resistance Exercise	Old individuals (65-75y) studied before and after 6 weeks of unilateral resistance exercise training
Young Unilateral Exercise	Resistance Exercise	Young Individuals (18-30y) studied before and after 6 weeks unilateral resistance exercise training

Primary Outcome Measures

Name	Time	Method
Muscle Protein Synthesis	0-6 Weeks	Comparison of muscle protein synthesis between young and older individuals and in response to 6 weeks unilateral exercise training

Secondary Outcome Measures

Name	Time	Method
Muscle Sub fractions	0-6 week	Comparison of different sub fractions involved in muscle mass regulation between young and older individuals and in response unilateral exercise training

Trial Locations

Locations (1)

Royal Derby Hospital Medical School; 🇬🇧 Derby, United Kingdom