Application of Deuterated Water (D2O) to Define the Etiology of Musculoskeletal Decline in Ageing and the Efficacy of Nutritional Supplementation

Not Applicable

• Conditions: Healthy
• Interventions: Behavioral: Exercise

• Registration Number: NCT02505438
• Lead Sponsor: University of Nottingham

Brief Summary

Maintaining muscle mass and function is important in healthy ageing to maintain quality of life. Loss of muscle mass is major problem, as beyond the age \~50, muscle mass declines by \~1-2% a year. To prevent this loss, the investigators need to understand the mechanism regulating muscle mass as we age. In this project the investigators aim to determine these mechanisms using a new technique of heavy water ingestion, this allows measurement of multiple aspects of skeletal muscle mass metabolism e.g. protein synthesis, over long periods (6 weeks) as people go about their normal everyday activities. The investigators also aim to determine the influence of resistance exercise training on older muscle, as well as differences between men and women in muscle responses to exercise and nutrition HMB (beta-hydroxy-beta-methylbutyrate).

Detailed Description

Not available

Study Timeline

• Study Start: 2013-04
• Study First Submitted: 2015-05-08
• Study First Submitted QC: 2015-07-21
• Study First Posted: 2015-07-22
• Status Verified: 2015-12
• Primary Completion: 2015-12
• Last Update Submitted: 2015-12-08
• Last Update Posted: 2015-12-09

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: All
• Target Recruitment: 48

Inclusion Criteria

• Young (18-30y) and old (60-75y) individuals who are generally healthy and recreationally active

Exclusion Criteria

• Active cardiovascular disease
• Cerebrovascular disease including previous stroke, aneurysm (large vessel or intracranial)
• Respiratory disease including pulmonary hypertension or Chronic obstructive pulmonary disease, Hyper/ hypo parathyroidism, hyper/ hypothyroidism,
• Cushing's disease, diabetes
• Active inflammatory bowel disease
• Renal disease
• Malignancy
• Recent steroid treatment (within 6 mo), or hormone replacement therapy Clotting dysfunction
• Musculoskeletal or neurological disorders
• Any disease requiring long-term drug prescriptions, including statins

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Young Unilateral Exercise	Exercise	Young Individuals (18-30y) studied before and after 6 weeks unilateral resistance exercise training
Old Unilateral Exercise and Omega 3	Exercise	Old individuals (65-75y) studied before and after 6 weeks of unilateral resistance exercise training with Omega 3 supplementation
Old Unilateral Exercise and HMB	Exercise	Old individuals (65-75y) studied before and after 6 weeks of unilateral resistance exercise training with HMB (beta-hydroxy-beta-methylbutyrate) supplementation
Old Unilateral Exercise	Exercise	Old individuals (65-75y) studied before and after 6 weeks of unilateral resistance exercise training

Primary Outcome Measures

Name	Time	Method
Muscle Protein Synthesis	0-6 Weeks	Comparison of muscle protein synthesis between young and older individuals and in response to 6 weeks unilateral exercise training and supplementation
Muscle Strength	0-6 Weeks	Maximal voluntary contractions of the quadriceps will be performed (leg extension) in the trained and untrained legs. This will be performed at baseline and repeated throughout to monitor training progression

Trial Locations

Locations (1)

Royal Derby Hospital Medical School; 🇬🇧 Derby, United Kingdom